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Immunology Immunoglobulins Heavy Chain IgG

Donkey Anti-Rabbit IgG H&L (HRP) (ab16284)

Price and availability

137 366 ₸

Availability

Order now and get it on Thursday February 25, 2021

Donkey Anti-Rabbit IgG H&L (HRP) (ab16284)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Donkey Anti-Rabbit IgG H&L (HRP)
  • Conjugation: HRP
  • Host species: Donkey
  • Isotype: IgG
  • Suitable for: WB

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Overview

  • Product name

    Donkey Anti-Rabbit IgG H&L (HRP)
    See all IgG secondary antibodies
  • Host species

    Donkey
  • Target species

    Rabbit
  • Specificity

    Reacts with the heavy and light chains of the immunoglobulin molecule.
  • Tested applications

    Suitable for: WBmore details
  • Immunogen

    Synthetic peptide corresponding to IgG.

  • Conjugation

    HRP

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C.
  • Storage buffer

    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 50% Glycerol (glycerin, glycerine), 1% BSA
  • Concentration information loading...
  • Purity

    Protein G purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • General notes

    Part of the AbExcel range.
  • Research areas

    • Immunology
    • Immunoglobulins
    • Heavy Chain
    • IgG
    • Secondary antibodies
    • anti-Rabbit
    • IgG
    • Enzyme
    • HRP

Images

  • Western blot - Donkey Anti-Rabbit IgG H&L (HRP) (ab16284)
    Western blot - Donkey Anti-Rabbit IgG H&L (HRP) (ab16284)
    All lanes : Anti-beta Actin antibody - Loading Control (ab8227) at 1 µg/ml

    Lanes 1-6 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lysates/proteins at 10 µg per lane.

    Secondary
    Lanes 1 - 2 : Donkey polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP) (ab16284) at 1/2000 dilution
    Lanes 3 - 4 : Donkey polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP) (ab16284) at 1/10000 dilution
    Lanes 5 - 6 : Donkey polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP) (ab16284) at 1/20000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Exposure time : 150 seconds
  • ELISA - Donkey Anti-Rabbit IgG H&L (HRP) (ab16284)
    ELISA - Donkey Anti-Rabbit IgG H&L (HRP) (ab16284)

    Ab16284 was used at dilution 1/5000 with the primary antibody ab52903 in ELISA. See the review on ab52903.

  • Western blot - Donkey Anti-Rabbit IgG H&L (HRP) (ab16284)
    Western blot - Donkey Anti-Rabbit IgG H&L (HRP) (ab16284)
    Ab16284 was used at dilution 1/2000 with the primary antibody ab28379 in WB. See the review on ab28379.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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