DNA Quantification Assay Kit (Fluorometric) (ab252898)
Key features and details
- Detection method: Fluorescent
- Platform: Microplate
- Sample type: Adherent cells, Purified mitochondria, Suspension cells, Tissue, Whole Blood
- Sensitivity: 4 ng/ml
Overview
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Product name
DNA Quantification Assay Kit (Fluorometric) -
Detection method
Fluorescent -
Sample type
Tissue, Adherent cells, Suspension cells, Whole Blood, Purified mitochondria -
Sensitivity
4 ng/ml -
Range
0.4 ng - 150 ng -
Product overview
DNA Quantification Assay Kit (Fluorometric) (ab252898) provides a quick, specific, and easy method for the measurement of DNA concentrations in a wide variety of samples. In this assay, DNA specifically reacts with a probe producing a stable fluorescence signal (Ex/Em = 492/528 nm). The kit is simple to perform, specific, sensitive and high-throughput adaptable with a wide detection range (0.4-150 ng DNA) and a limit of detection of 4 pg/µL dsDNA in samples.
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Platform
Microplate
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 2000 tests 10X DNA Buffer 1 x 50ml 200X DNA Probe (in DMSO) 1 x 1ml Lambda DNA Standard 1 x 10ml
Images
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Lambda DNA standard curve.
Lambda DNA standard curve.
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Assay specificity.The probe specifically recognizes dsDNA only. ssDNA and RNA are not detected.
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Effect of contaminants.Lambda DNA (150 ng/well) mixed with different concentrations of inorganic and organic contaminants commonly found in DNA samples; ([contaminants] based on 10 µL of DNA sample/well).