DAF-FM DA, Nitric oxide quantification reagent (ab145295)
Key features and details
- Cell permeable reagent for quantification of low concentrations of nitric oxide in solution.
- CAS Number: 254109-22-3
- Purity: > 98%
- Soluble in DMSO
- Form / State: Solid
- Source: Synthetic
Overview
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Product name
DAF-FM DA, Nitric oxide quantification reagent -
Description
Cell permeable reagent for quantification of low concentrations of nitric oxide in solution. -
Alternative names
- 4-Amino-5-methylamino- 2',7'-difluorofluorescein diacetate
- DAF-FM diacetate
- Diaminofluorescein-FM diacetate
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Biological description
Cell permeable reagent for quantification of low concentrations of nitric oxide in solution. Essentially nonfluorescent until it is hydrolyzed to DAF-FM (ab145297) by intracellular esterases and reacts with NO to form a fluorescent benzotriazole.
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Purity
> 98% -
CAS Number
254109-22-3 -
Chemical structure
Properties
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Excitation
500nm -
Emission
515nm -
Chemical name
3-Amino-4-(N-methylamino)-2',7'-difluorofluorescein diacetate -
Molecular weight
496.42 -
Molecular formula
C25H18F2N2O7 -
Storage instructions
Store at -20°C. Store under desiccating conditions. The product can be stored for up to 12 months. -
Solubility overview
Soluble in DMSO -
Handling
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.
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Source
Synthetic