D-Amino Acid Oxidase Activity Assay Kit (Fluorometric) (ab273325)
Key features and details
- Assay type: Enzyme activity (quantitative)
- Detection method: Fluorescent
- Platform: Microplate
- Sample type: Tissue
- Sensitivity: 0.5 µU
Overview
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Product name
D-Amino Acid Oxidase Activity Assay Kit (Fluorometric) -
Detection method
Fluorescent -
Sample type
Tissue -
Assay type
Enzyme activity (quantitative) -
Sensitivity
0.5 µU -
Assay duration
Multiple steps standard assay -
Product overview
D-Amino Acid Oxidase Activity Assay Kit (Fluorometric) (ab273325) provides a quick and easy method for monitoring D-Amino Acid Oxidas (DAAO) activities in a wide variety of samples. In this assay, DAAO converts D-amino acid into hydrogen peroxide as one byproduct. Consequently, the formed byproduct will react with a probe producing a strong fluorescent signal (Ex/Em= 535/587nm).
The kit is simple, sensitive, high-throughput adaptable and can detect as low as 0.5 µU of DAAO activity.
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Platform
Microplate
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 100 tests DAAO Assay Buffer 1 x 25ml DAAO Cofactor (lyophilized) 1 vial DAAO Enzyme Mix (lyophilized) 1 vial DAAO Positive Control (lyophilized) 1 vial DAAO Probe (in DMSO) 1 x 200µl DAAO Substrate 1 x 220µl H2O2 Standard (0.88 M) 1 x 100µl -
Research areas
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Function
Regulates the level of the neuromodulator D-serine in the brain. Has high activity towards D-DOPA and contributes to dopamine synthesis. Could act as a detoxifying agent which removes D-amino acids accumulated during aging. Acts on a variety of D-amino acids with a preference for those having small hydrophobic side chains followed by those bearing polar, aromatic, and basic groups. Does not act on acidic amino acids. -
Sequence similarities
Belongs to the DAMOX/DASOX family. -
Cellular localization
Peroxisome. - Information by UniProt
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Alternative names
- D-amino-acid oxidase
- DAAO
- DAMOX
see all
Images
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H2O2 Standard curve
Typical standard curve – data provided for demonstration purposes only. A new standard curve must be generated for each assay performed.
H2O2 Standard curve.
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DAAO activities in Rat tissuesExample data.
DAAO activities in perfused rat kidney (0.447 µg protein), rat cerebellum (8.67 µg protein).
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Specific DAAO activities in Rat tissuesExample data.
Specific DAAO activities in perfused rat kidney and rat cerebellum. Assays were performed following the kit protocol.
