Cytohesin 2 overexpression 293T lysate (whole cell) (ab94253)
Overview
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Product name
Cytohesin 2 overexpression 293T lysate (whole cell) -
General notes
ab94253 is a 293T cell transfected lysate in which Human Cytohesin 2 has been transiently over-expressed using a pCMV-Cytohesin 2 plasmid. The lysate is provided in 1X Sample Buffer. Note: For more details about how the transfected lysate was prepared view preparation notes
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Tested applications
Suitable for: WBmore details
Properties
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Mycoplasma free
Yes -
Form
Liquid -
Storage instructions
Shipped on dry ice. Upon delivery aliquot and store at -20ºC. Avoid freeze / thaw cycles. -
Storage buffer
Constituents: 0.01% Bromophenol blue, 2.3% Beta mercaptoethanol, 2% Sodium lauryl sulfate, 0.788% Tris HCl, 10% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Research areas
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Background
Function: Acts as a guanine-nucleotide exchange factor (GEF). Promotes guanine-nucleotide exchange on ARF1, ARF3 and ARF6. Promotes the activation of ARF factors through replacement of GDP with GTP. The cell membrane form, in association with ARL4 proteins, recruits ARF6 to the plasma membrane. Tissue specificity: Ubiquitous. Similarity: Contains 1 PH domain. Contains 1 SEC7 domain. Domain: The PH domain is necessary and sufficient for plasma membrane relocalization.
Images
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SDS-PAGE - Cytohesin 2 overexpression 293T lysate (whole cell) (ab94253)ab94253 at 15μg/lane on an SDS-PAGE gel
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Western blot - Cytohesin 2 overexpression 293T lysate (whole cell) (ab94253)All lanes : Anti-Cytohesin 2 antibody (ab56510) at 1/500 dilution
Lane 1 :Cytohesin 2 overexpression 293T lysate (whole cell) (ab94253)
Lane 2 : 293T non-transfected lysate
Lysates/proteins at 25 µg per lane.
Secondary
All lanes : Goat Anti-mouse IgG (H and L) HRP conjugated at 1/2500 dilution
