Cholesterol/Cholesteryl Ester Assay Kit - Detection (ab102515)
Key features and details
- Assay type: Quantitative
- Detection method: Colorimetric
- Platform: Microplate reader
- Assay time: 1 hr
- Sample type: Cell Lysate, Tissue Lysate
Overview
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Product name
Cholesterol/Cholesteryl Ester Assay Kit - Detection
See all Cholesterol/Cholesteryl Ester kits -
Detection method
Colorimetric -
Sample type
Cell Lysate, Tissue Lysate -
Assay type
Quantitative -
Assay time
1h 00m -
Product overview
Cholesterol/Cholesteryl Ester Assay Kit ab102515 uses a simple method for sensitive quantification of free cholesterol, cholesteryl esters, or both using a colorimetric method.
This assay kit uses an alternative method compared to our most popular Cholesterol/ Cholesteryl Ester Assay Kit ab65359 (which relies on a cholesterol oxidase based method).
In this assay kit, free cholesterol is oxidized by cholesterol dehydrogenase to generate NADH which reacts with a sensitive probe resulting in strong absorbance at 450 nm.
The assay can detect free or total cholesterol depending upon whether cholesterol esterase is used to hydrolyze cholesterol esters present.
Cholesteryl ester can be determined by subtracting the value of free cholesterol from the total cholesterol (cholesterol plus cholesteryl esters).
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Notes
Cholesterol is an essential molecule in all animal life. It has been involved in both normal development and diseases.
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses. -
Platform
Microplate reader
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components Identifier 100 tests Cholesterol Assay Buffer WM 1 x 25ml Cholesterol Standard Yellow 1 x 100µl Enzyme Mix (lyophilized) Green 1 vial Esterase (lyophilized) Blue 1 vial Substrate Mix (lyophilized) Red 1 vial -
Research areas
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Relevance
Cholesterol is an essential molecule in all animal life. It is involved in both normal development and diseases. A high level of serum cholesterol is an indicator for diseases such as heart disease. Cholesteryl ester is an ester of cholesterol. The ester bond is formed between the carboxylate group of a fatty acid and the hydroxyl group of cholesterol.
Images
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Functional Studies - Cholesterol/Cholesteryl Ester Detection Kit (ab102515)Cholesterol Standard Curve: Cholesterol/Cholesteryl Ester was quantified according to the kit instructions. Background from the 0 standard reading (without cholesterol) has been subtracted from all readings.
