Chloroplast Isolation Kit (ab234623)
Overview
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Product name
Chloroplast Isolation Kit -
Product overview
Chloroplast Isolation Kit (ab234623) provides an easy method to isolate chloroplasts from a broad range of plant types including dicots, monocots, and conifers. The isolated chloroplasts can be used to study photosynthetic processes and as starting materials for chloroplast membrane, protein, chloroplast DNA and chloroplast RNA isolations.
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Notes
Chloroplasts are membrane-bound organelles found in plant and algal cells which perform the function of photosynthesis. Plant chloroplasts are lens-shaped, usually 5-10 µm in diameter and 1-3 µm thick. Although leaves contain the majority of plants’ chloroplasts, they can also be found in stems, seeds, and un-ripened fruit.
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 10 g BSA (10% solution) 1 x 10ml Chloroplast Isolation Buffer, 2X 1 x 500ml Density Gradient Medium 1 x 50ml DTT (1 M) 1 x 1ml
Images
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Light microscopy of chloroplasts isolated from Ficus leaves
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Representation of Density Gradient isolation of intact chloroplasts -
Chlorophyll a content at each stage of the chloroplast isolation process.(a) Debris pellet from first centrifugation at step 7.1.6; (b) Supernatant from step 7.1.7; (c) Intact chloroplasts from step 7.2.3; (d) Broken chloroplasts from step 7.2.3.
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. Chlorophyll a content of isolated chloroplasts from a range of plants(a) Spider plant (Chlorophytum comosim); (b) Corn husks (Zea mays); (c) Pine needles (Pinus muricata); (d) Cypress needles (Cupressus sempervirens); (e) Juniper (Juniperus species); (f) Lettuce (Lactuca sativa); (g) Stapelia variegata; (h) Ficus species.