Chloride Channel Assay Kit (Colorimetric) (ab176767)
Key features and details
- Assay type: Quantitative
- Detection method: Colorimetric
- Platform: Microplate reader
- Sample type: Adherent cells, Suspension cells
Overview
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Product name
Chloride Channel Assay Kit (Colorimetric) -
Detection method
Colorimetric -
Sample type
Adherent cells, Suspension cells -
Assay type
Quantitative -
Product overview
Abcam’s Chloride Channel Assay Kit (Colorimetric) (ab176767) provides a sensitive and robust colorimetric method for studying chloride channels. The assay is based on our proprietary iodide indicator (Iodide Sensor Blue dye) to measure iodide concentration, as low as 30 nM of iodide can be detected. Iodide Sensor Blue dye forms a blue complex with iodide, which has absorption spanning from the UV to 700 nm. Thus a few absorption wavelengths can be used for monitoring the iodide-dependent color change.
Abcam's Chloride Channel Assay Kit (Colorimetric) (ab176767) provides an optimized assay method for monitoring chloride channels. The assay can be performed in a convenient 96-well or 384-well microtiter-plate format.
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Notes
Chloride channels have a variety of important physiological and cellular functions that include regulation of pH, volume homeostasis, organic solute transport, cell migration, cell proliferation and differentiation. Chloride channels represent valuable drug targets. A number of chronic disease states such as cystic fibrosis and Bartter's syndrome are due to defects in chloride channel functions. However, the existing technologies for screening chloride channel modulators are a compromise between throughput, sensitivity and physiological relevance.
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Platform
Microplate reader
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 10 x 96 tests 100X Iodide Sensor Enhancer 1 x 0.5ml 10X Cell Lysis Buffer 1 x 5ml I-Loading Buffer 1 x 100ml Iodide Sensor Blue dye 1 x 50ml -
Research areas
Images
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Dose response curve for Chloride Channel Assay Kit (Colorimetric) (ab176767)
Sodium iodide (Nal) dose response was measured with Abcam's Chloride Channel Assay Kit (Colorimetric) (ab176767) in a 96-well black wall/clear bottom plate. As low as 30 nM of Nal was detected with 10 minutes incubation time (n = 3).
