Astana Biomed Group, an authorized Abcam distributor in Central Asia

Chicken IgY ELISA Kit (ab189577)

Key features and details

  • One-wash 90 minute protocol
  • Sensitivity: 14 pg/ml
  • Range: 0.16 ng/ml - 10 ng/ml
  • Sample type: Cell culture supernatant, Cit plasma, EDTA Plasma, Hep Plasma, Serum
  • Detection method: Colorimetric
  • Assay type: Sandwich (quantitative)
  • Reacts with: Chicken

Overview

  • Product name

    Chicken IgY ELISA Kit
    See all IgY kits

  • Detection method

    Colorimetric

  • Precision

    Intra-assay
    Sample n Mean SD CV%
    Overall 3 2.2%
    Inter-assay
    Sample n Mean SD CV%
    Overall 3 4.49%

  • Sample type

    Cell culture supernatant, Serum, Hep Plasma, EDTA Plasma, Cit plasma

  • Assay type

    Sandwich (quantitative)

  • Sensitivity

    14 pg/ml

  • Range

    0.16 ng/ml - 10 ng/ml

  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Antibody Diluent 4B 103.18 99.6% - 105.68%
    1X Cell Extraction Buffer PTR 98.48 97.68% - 100.53%
    1X Wash Buffer 98.34 87.31% - 104.46%
    OF DMEM Medium 62.52 52.05% - 76.75%
    10F DMEM Medium 75.89 70.17% - 86.63%

  • Assay time

    1h 30m

  • Assay duration

    One step assay

  • Species reactivity

    Reacts with: Chicken
    Does not react with: Goat, Cow, Pig

  • Product overview

    Chicken IgY ELISA Kit (ab189577) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of IgY protein in cell culture supernatant, cit plasma, edta plasma, hep plasma, and serum. It uses our proprietary SimpleStep ELISA® technology. Quantitate Chicken IgY with 14 pg/ml sensitivity.


    SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:


            - Single-wash protocol reduces assay time to 90 minutes or less
            - High sensitivity, specificity and reproducibility from superior antibodies
            - Fully validated in biological samples
            - 96-wells plate breakable into 12 x 8 wells strips


    A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

  • Notes

    In chickens, immunoglobulin Y (IgY) is the functional equivalent to Immunoglobulin G (IgG). Like IgG, it is composed of two light and two heavy chains. Structurally, these two types of immunoglobulin differ primarily in the heavy chains, which in IgY have a molecular mass of about 65,100 atomic mass units (amu), and are thus larger than in IgG. The light chains in IgY, with a molar mass of about 18,700 amu, are somewhat smaller than the light chains in IgG. The molar mass of IgY thus amounts to about 167,000 amu. The steric flexibility of the IgY molecule is less than that of IgG.

    Functionally, IgY is partially comparable to Immunoglobulin E (IgE), as well as to IgG. However, in contrast to IgG, IgY does not bind to Protein A, to Protein G, or to cellular Fc receptors. Furthermore, IgY does not activate the complement system.

    Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
    It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

  • Platform

    Microplate

Properties

  • Storage instructions

    Store at +4°C. Please refer to protocols.
  • Components 1 x 96 tests
    10X Chicken IgY Capture Antibody 1 x 600µl
    10X Chicken IgY Detector Antibody 1 x 600µl
    10X Wash Buffer PT (ab206977) 1 x 20ml
    50X Cell Extraction Enhancer Solution (ab193971) 1 x 1ml
    5X Cell Extraction Buffer PTR (ab193970) 1 x 10ml
    Antibody Diluent 4B 1 x 6ml
    Chicken IgY Lyophilized Purified Protein 2 vials
    Plate Seals 1 unit
    Sample Diluent NS (ab193972) 1 x 50ml
    SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit
    Stop Solution 1 x 12ml
    TMB Development Solution 1 x 12ml

  • Relevance

    Secreted immunoglobulins have two separable functions: one is to bind specifically to pathogenic molecules, the other is to recruit cells and molecules to destroy the opsonized pathogen. IgY has a tetrameric structure consisting of two identical light chains (Mr = 24 kDa) and two identical heavy chains (Mr = 73 kDa).

  • Alternative names

    • FCR
    • IGSF1
    • immunoglobulin superfamily, member 1

  • Database links

    Images

    • Other - Chicken IgY ELISA Kit (ab189577)
      Other - Chicken IgY ELISA Kit (ab189577)

      SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.

       

    • Example of Chicken IgY standard curve.
      Example of Chicken IgY standard curve.

      Background-subtracted data values (mean +/- SD) are graphed.

    • Example of chicken serum IgY level in Sample Diluent NS.
      Example of chicken serum IgY level in Sample Diluent NS.

      Interpolated chicken IgY value are graphed.

    • Example of chicken egg yolk IgY level in Sample Diluent NS.
      Example of chicken egg yolk IgY level in Sample Diluent NS.

      Interpolated chicken IgY value are graphed.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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