Chicken IFN-alpha ELISA Kit (ab273260)
Key features and details
- Sensitivity: 22 pg/ml
- Range: 20.48 pg/ml - 5000 pg/ml
- Sample type: Cell culture supernatant, EDTA Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Chicken
Overview
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Product name
Chicken IFN-alpha ELISA Kit
See all IFN alpha kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Overall Inter-assay Sample n Mean SD CV% Overall -
Sample type
Cell culture supernatant, Serum, EDTA Plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
22 pg/ml -
Range
20.48 pg/ml - 5000 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Serum 104.9 73% - 134% Plasma 84.98 67% - 106% Cell culture media 93.02 76% - 113% -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Chicken -
Product overview
Chicken IFN alpha ELISA Kit (ab273260) is an in-vitro enzyme-linked immunosorbent assay for the quantitative measurement of Chicken IFN alpha in serum (Chicken IFN-alpha concentration is pretty low in normal serum/plasma, it may not be detected in this assay), plasma EDTA (Citrate and Heparin have not been validated yet) and cell culture supernatants.
This assay employs an antibody specific for Chicken IFN alpha coated on a 96-well plate. Standards and samples are pipetted into the wells and IFN alpha present in a sample is bound to the wells by the immobilized antibody. The wells are washed, and biotinylated anti-Chicken IFN alpha antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IFN alpha bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
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Tested applications
Suitable for: Sandwich ELISAmore details -
Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 1 x 96 tests 20X Wash Buffer 1 x 25ml 5X Assay Diluent B 1 x 15ml 5X Assay Diluent D 1 x 15ml 600X HRP-Streptavidin Concentrate 1 x 200µl Anti-Chicken IFN-alpha coated Microplate (12 x 8 wells) 1 unit Biotinylated Anti-Chicken IFN-alpha Antibody 2 vials Chicken IFN-alpha Standard (Lyophilized) 2 vials Stop Solution 1 x 8ml TMB Substrate Solution 1 x 12ml -
Research areas
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Relevance
A member of the type 1 IFN family of related proteins produced by leucocytes and fibroblasts. IFN alpha 1 has antiviral activities. Interferon stimulates the production of two enzymes: a protein kinase and an oligoadenylate synthetase. -
Cellular localization
Secreted -
Alternative names
- IFL
- IFN
- IFN alpha
see all
Images
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Chicken IFNalpha ELISA Standard Curve
This standard curve is for demonstration only. A standard curve must be run with each assay.