Ceruloplasmin Activity Assay Kit (Colorimetric) (ab273296)
Key features and details
- Assay type: Enzyme activity (quantitative)
- Detection method: Colorimetric
- Platform: Microplate
- Sample type: Serum
Overview
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Product name
Ceruloplasmin Activity Assay Kit (Colorimetric)
See all Ceruloplasmin kits -
Detection method
Colorimetric -
Sample type
Serum -
Assay type
Enzyme activity (quantitative) -
Range
5 mU/ml - 500 mU/ml -
Assay duration
Multiple steps standard assay -
Product overview
Ceruloplasmin Activity Assay Kit (Colorimetric) (ab273296) utilizes an oxidase substrate, which gives an intensely colored product upon oxidation. The kit is fast, easy to use and suitable for high throughput applications and can measure ceruloplasmin activity in serum in the range between approximately 5 and 500 mU/ml.
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Platform
Microplate
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 100 tests Ammonium Sulfate, saturated (~4.1M) 1 x 10ml Ceruloplasmin Assay Buffer 1 x 25ml Ceruloplasmin Substrate 1 x 1ml Oxidizer (100 mM) 1 x 100µl Stabilizer 1 x 100µl -
Research areas
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Function
Ceruloplasmin is a blue, copper-binding (6-7 atoms per molecule) glycoprotein. It has ferroxidase activity oxidizing Fe(2+) to Fe(3+) without releasing radical oxygen species. It is involved in iron transport across the cell membrane. -
Tissue specificity
Expressed by the liver and secreted in plasma. -
Involvement in disease
Defects in CP are the cause of aceruloplasminemia (ACERULOP) [MIM:604290]. It is an autosomal recessive disorder of iron metabolism characterized by iron accumulation in the brain as well as visceral organs. Clinical features consist of the triad of retinal degeneration, diabetes mellitus and neurological disturbances.
Note=Ceruloplasmin levels are decreased in Wilson disease, in which copper cannot be incorporated into ceruloplasmin in liver because of defects in the copper-transporting ATPase 2. -
Sequence similarities
Belongs to the multicopper oxidase family.
Contains 3 F5/8 type A domains.
Contains 6 plastocyanin-like domains. -
Cellular localization
Secreted. - Information by UniProt
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Alternative names
- CERU_HUMAN
- Ceruloplasmin
- CP
see all
Images
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Typical Standard Curve of chemically oxidized substrate.
Typical standard curve – data provided for demonstration purposes only. A new standard curve must be generated for each assay performed.
Typical Standard Curve of chemically oxidized substrate. The Standard Curve slope (for the points below 1.0 OD) is 0.0214 OD/nmol.
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Ceruloplasmin Activity AssayKinetic curves obtained from varying sample volumes of human serum. The kinetic slope for a 10 μl sample of an Ammonium Sulfate precipitate of frozen pooled human serum is 0.0189. Serum Ceruloplasmin Activity = 0.0189 (OD/min)/0.0214 (OD/nmol)/0.01ml X 2 = 176 mU/ml.