Cell Tracking Dye Kit - Orange - Cytopainter (ab138892)
Overview
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Product name
Cell Tracking Dye Kit - Orange - Cytopainter
See all Cell tracking kits -
Detection method
Fluorescent -
Sample type
Adherent cells, Suspension cells -
Assay type
Cell-based -
Product overview
Cell Tracking Dye Kit - Orange | Cytopainter (ab138892) is designed to uniformly label live cells for studies where multicolor analysis might be required, such as cell drug resistance, cell adhesion or chemotaxis. Dye can be visualized with an orange filter (Ex/Em = 540/560 nm).
The labelling process is robust, requiring minimal hands-on time. This product can be used in different types of fluorescence platforms such as microplate assays, flow cytometry and fluorescence microscope.The kit provides all the essential components with an optimized cell-labelling protocol, and can be used for both proliferating and non-proliferating cells (either suspension or adherent cells).
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Notes
Uniform labeling of live cells with a hydrophobic dye whose fluorescence is strongly enhanced upon entering the cell. Cellular esterases convert the substrate into a strong fluorescent photostable hydrophilic dye that is well retained in the cytoplasm. Cells need to be viewed in less than 24 - 48 hours as dye will not be passed to daughter cells.
If you would like to track cells during several generations, we recommend CytoPainter Cell Proliferation Staining Reagent - Orange Fluorescence (ab176737).
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Platform
Microplate reader, Fluor. microscope, Flow cyt.
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 200 tests Assay Buffer 1 x 20ml Tracking dye Orange 1 x 50µl -
Research areas
Images
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Fluorescence MicroscopyImage of Hela cells stained with 1X CytoPainter Cell Tracking Staining Kit - Orange Fluorescence in a black wall/clear bottom 96-well plate.
