Carboxylesterase 1 (CES1) Specific Activity Assay Kit (ab109717)
Key features and details
- Assay type: Enzyme activity
- Detection method: Colorimetric
- Platform: Microplate reader
- Sample type: Cell culture extracts, Tissue
Overview
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Product name
Carboxylesterase 1 (CES1) Specific Activity Assay Kit
See all Carboxylesterase 1 kits -
Detection method
Colorimetric -
Sample type
Cell culture extracts, Tissue -
Assay type
Enzyme activity -
Species reactivity
Reacts with: Rat, Human -
Product overview
ab109717 (MS789) is a novel assay that uses a high affinity monoclonal capture antibody to selectively isolate carboxylesterase 1 (CES1) from liver tissue or cell samples. After isolation and subsequent measurement of the enzyme's functional activity, the quantity of isolated CES1 is measured in the same well by adding a second monoclonal detector antibody, which is quantified using a colorimetric label (HRP). Both reactions take place in time-dependent manners proportional to the amount of enzyme captured in each well. By combining activity and quantity measurements, the enzyme's relative specific activity can be determined. Specific activity is useful for measuring up or down regulation of activity by site-specific modification or damage, and in response to specific inhibitors.
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Notes
All components are shipped cold. Store all components at 4°C.
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Platform
Microplate reader
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 100X HRP Label 1 x 125µl 10X Blocking Solution 1 x 10ml 1X Base Buffer 1 x 25ml 20X Wash Buffer 1 x 25ml 5700X 4-nitrophenol 1 x 20µl CES1 antibody precoated Microtiter plate 1 unit CES1 Detector antibody 1 x 125µl Extraction Buffer (ab260490) 1 x 15ml HRP Development Solution 1 x 12ml -
Research areas
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Alternative names
- ACAT
- Acyl coenzyme A:cholesterol acyltransferase
- Brain carboxylesterase hBr1
see all -
Database links
- SwissProt: P23141 Human
Images
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- Carboxylesterase 1 (CES1) Specific Activity Assay Kit (ab109717)Figure 1. Using this assay the acetylcholinesterase inhibitor tacrine, previosuly shown to co-crystalize in the active site of CES1, does not inhibit the activity of the enzyme which is in agreement with the crystalization study (Bencharit et al. Chem Biol 2003; 10(4) 341-9). Troglitazone a member of the thiazolidinediones class of antidiabetic drugs, was determined to inhihibit CES1 (IC50 3µM) while other members of this class had little/no effect (Fukami et al. Drug Metab Dispos 2010; 38(12):2173-8.
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Sandwich ELISA - Carboxylesterase 1 (CES1) Specific Activity Assay Kit (ab109717)Principle of Sandwich ELISA used in Carboxylesterase 1 (CES1) Specific Activity Assay Kit (ab109717). Substrate plus HRP or AP conjugated to streptavidin provide a colorimetric signal that is readable by any plate readers capable of standard ELISA absorbance measurements.