Canine TIMP2 ELISA Kit (ab273176)
Key features and details
- Sensitivity: 20 pg/ml
- Range: 20.48 pg/ml - 5000 pg/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Dog
Overview
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Product name
Canine TIMP2 ELISA Kit
See all TIMP2 kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Overall Inter-assay Sample n Mean SD CV% Overall -
Sample type
Cell culture supernatant, Serum, Plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
20 pg/ml -
Range
20.48 pg/ml - 5000 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Serum 125.9 109% - 142% Plasma 120.4 107% - 141% Cell culture media 123.6 102% - 144% -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Dog -
Product overview
Canine TIMP2 ELISA Kit (ab273176) is an in-vitro enzyme-linked immunosorbent assay for the quantitative measurement of Canine TIMP2 in serum, plasma and cell culture supernatants.
This assay employs an antibody specific for Canine TIMP2 coated on a 96-well plate. Standards and samples are pipetted into the wells and Canine TIMP2 present in a sample is bound to the wells by the immobilized antibody. The wells are washed, and biotinylated anti-Canine TIMP2 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Canine TIMP2 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
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Tested applications
Suitable for: Sandwich ELISAmore details -
Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 1 x 96 tests 20X Wash Buffer 1 x 25ml 500X HRP-Streptavidin Concentrate 1 x 200µl 5X Assay Diluent 1 x 15ml Anti-Canine TIMP2 coated Microplate (12 x 8 wells) 1 unit Biotinylated Anti-Canine TIMP2 Antibody 2 vials Canine TIMP2 Standard (Lyophilized) 2 vials Stop Solution 1 x 8ml TMB Substrate Solution 1 x 12ml -
Research areas
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Function
Complexes with metalloproteinases (such as collagenases) and irreversibly inactivates them by binding to their catalytic zinc cofactor. Known to act on MMP-1, MMP-2, MMP-3, MMP-7, MMP-8, MMP-9, MMP-10, MMP-13, MMP-14, MMP-15, MMP-16 and MMP-19. -
Sequence similarities
Belongs to the protease inhibitor I35 (TIMP) family.
Contains 1 NTR domain. -
Post-translational
modificationsThe activity of TIMP2 is dependent on the presence of disulfide bonds. -
Cellular localization
Secreted. - Information by UniProt
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Alternative names
- CSC 21K
- CSC-21K
- CSC21K
see all -
Database links
- Entrez Gene: 403633 Dog
- SwissProt: Q9TTY1 Dog
Images
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Canine TIMP2 ELISA Kit standard curve
This standard curve is for demonstration only. A standard curve must be run with each assay.