Canine IL-2 ELISA Kit (ab273172)
Key features and details
- Sensitivity: 0.4 ng/ml
- Range: 0.41 ng/ml - 100 ng/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Dog
Overview
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Product name
Canine IL-2 ELISA Kit
See all IL-2 kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Overall Inter-assay Sample n Mean SD CV% Overall -
Sample type
Cell culture supernatant, Serum, Plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
0.4 ng/ml -
Range
0.41 ng/ml - 100 ng/ml -
Recovery
Sample specific recovery Sample type Average % Range Cell culture supernatant 92.16 73% - 114% Serum 96.12 77% - 110% Plasma 84.77 68% - 102% -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Dog -
Product overview
Canine IL-2 ELISA Kit (ab273172) is an in-vitro enzyme-linked immunosorbent assay for the quantitative measurement of Canine IL-2 in serum (Canine IL-2 concentration is low in normal serum/plasma, and may not be detectable in this assay), plasma and cell culture supernatants.
This assay employs an antibody specific for Canine IL-2 coated on a 96-well plate. Standards and samples are pipetted into the wells and Canine IL-2 present in a sample is bound to the wells by the immobilized antibody. The wells are washed, and biotinylated anti-Canine IL-2 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Canine IL-2 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
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Tested applications
Suitable for: Sandwich ELISAmore details -
Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 1 x 96 tests 20X Wash Buffer 1 x 25ml 500X HRP-Streptavidin Concentrate 1 x 200µl 5X Assay Diluent B 1 x 15ml 5X Assay Diluent D 1 x 15ml Anti-Canine IL-2 coated Microplate (12 x 8 wells) 1 unit Biotinylated Anti-Canine IL-2 Antibody 2 vials Canine IL-2 Standard (Lyophilized) 2 vials Stop Solution 1 x 8ml TMB Substrate Solution 1 x 12ml -
Research areas
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Function
Produced by T-cells in response to antigenic or mitogenic stimulation, this protein is required for T-cell proliferation and other activities crucial to regulation of the immune response. Can stimulate B-cells, monocytes, lymphokine-activated killer cells, natural killer cells, and glioma cells. -
Involvement in disease
Note=A chromosomal aberration involving IL2 is found in a form of T-cell acute lymphoblastic leukemia (T-ALL). Translocation t(4;16)(q26;p13) with involves TNFRSF17. -
Sequence similarities
Belongs to the IL-2 family. -
Cellular localization
Secreted. - Information by UniProt
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Alternative names
- Aldesleukin
- IL 2
- IL-2
see all -
Database links
- Entrez Gene: 403989 Dog
- SwissProt: Q29416 Dog
Images
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Canine IL-2 ELISA Kit standard curve
This standard curve is for demonstration only. A standard curve must be run with each assay.
