Astana Biomed Group, an authorized Abcam distributor in Central Asia

Canine IL-2 ELISA Kit (ab273172)

Key features and details

  • Sensitivity: 0.4 ng/ml
  • Range: 0.41 ng/ml - 100 ng/ml
  • Sample type: Cell culture supernatant, Plasma, Serum
  • Detection method: Colorimetric
  • Assay type: Sandwich (quantitative)
  • Reacts with: Dog

Overview

  • Product name

    Canine IL-2 ELISA Kit
    See all IL-2 kits

  • Detection method

    Colorimetric

  • Precision

    Intra-assay
    Sample n Mean SD CV%
    Overall
    Inter-assay
    Sample n Mean SD CV%
    Overall

  • Sample type

    Cell culture supernatant, Serum, Plasma

  • Assay type

    Sandwich (quantitative)

  • Sensitivity

    0.4 ng/ml

  • Range

    0.41 ng/ml - 100 ng/ml

  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 92.16 73% - 114%
    Serum 96.12 77% - 110%
    Plasma 84.77 68% - 102%

  • Assay duration

    Multiple steps standard assay

  • Species reactivity

    Reacts with: Dog

  • Product overview

    Canine IL-2 ELISA Kit (ab273172) is an in-vitro enzyme-linked immunosorbent assay for the quantitative measurement of Canine IL-2 in serum (Canine IL-2 concentration is low in normal serum/plasma, and may not be detectable in this assay), plasma and cell culture supernatants.


    This assay employs an antibody specific for Canine IL-2 coated on a 96-well plate. Standards and samples are pipetted into the wells and Canine IL-2 present in a sample is bound to the wells by the immobilized antibody. The wells are washed, and biotinylated anti-Canine IL-2 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Canine IL-2 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • Tested applications

    Suitable for: Sandwich ELISAmore details

  • Platform

    Pre-coated microplate (12 x 8 well strips)

Properties

  • Storage instructions

    Store at -20°C. Please refer to protocols.
  • Components 1 x 96 tests
    20X Wash Buffer 1 x 25ml
    500X HRP-Streptavidin Concentrate 1 x 200µl
    5X Assay Diluent B 1 x 15ml
    5X Assay Diluent D 1 x 15ml
    Anti-Canine IL-2 coated Microplate (12 x 8 wells) 1 unit
    Biotinylated Anti-Canine IL-2 Antibody 2 vials
    Canine IL-2 Standard (Lyophilized) 2 vials
    Stop Solution 1 x 8ml
    TMB Substrate Solution 1 x 12ml

  • Research areas

  • Function

    Produced by T-cells in response to antigenic or mitogenic stimulation, this protein is required for T-cell proliferation and other activities crucial to regulation of the immune response. Can stimulate B-cells, monocytes, lymphokine-activated killer cells, natural killer cells, and glioma cells.

  • Involvement in disease

    Note=A chromosomal aberration involving IL2 is found in a form of T-cell acute lymphoblastic leukemia (T-ALL). Translocation t(4;16)(q26;p13) with involves TNFRSF17.

  • Sequence similarities

    Belongs to the IL-2 family.

  • Cellular localization

    Secreted.
  • Information by UniProt

  • Alternative names

    • Aldesleukin
    • IL 2
    • IL-2
    • IL2
    • IL2_HUMAN
    • Interleukin 2
    • Interleukin-2
    • interleukin2
    • Involved in regulation of T cell clonal expansion
    • Lymphokine
    • OTTHUMP00000164090
    • POIL2
    • T Cell Growth Factor
    • T-cell growth factor
    • TCGF
    see all

  • Database links

    Images

    • Canine IL-2 ELISA Kit standard curve
      Canine IL-2 ELISA Kit standard curve

      This standard curve is for demonstration only. A standard curve must be run with each assay.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

    © 2021 Astana Biomed Group LLP. All rights reserved.