Canine IL-10 ELISA Kit (ab193685)
Key features and details
- Sensitivity: 0.1 ng/ml
- Range: 0.1 ng/well - 80 ng/well
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Dog
Overview
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Product name
Canine IL-10 ELISA Kit
See all IL-10 kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Overall Inter-assay Sample n Mean SD CV% Overall -
Sample type
Cell culture supernatant, Serum, Plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
0.1 ng/ml -
Range
0.1 ng/well - 80 ng/well -
Recovery
Sample specific recovery Sample type Average % Range Serum 116.3 94% - 138% Plasma 120.1 93% - 149% Cell culture media 95.6 77% - 115% -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Dog -
Product overview
Abcam’s IL-10 Canine ELISA Kit (ab193685) is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of canine IL-10 in serum, plasma and cell culture supernatants.
This assay employs an antibody specific for canine IL-10 coated on a 96-well plate. Standards and samples are pipetted into the wells and the immobilized antibody captures IL-10 present in the samples. The wells are washed and biotinylated anti-canine IL-10 antibody is added. After washing away any unbound biotinylated antibody, an HRP-conjugated streptavidin is pipetted to the wells. After incubation, the wells are again washed, followed by the addition of a TMB substrate solution to the wells. Color will develop in proportion to the amount of IL-10 bound in each well. Addition of the Stop Solution will change the color from blue to yellow, and the intensity of the color is measured at 450 nm.
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Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 1 x 96 tests 20X Wash Buffer Concentrate 1 x 25ml 350X HRP-Streptavidin Concentrate 1 x 200µl 5X Assay Diluent D 1 x 15ml 5X Assay Diluent B 1 x 15ml Biotinylated Canine IL-10 Detection Antibody 2 vials Canine IL-10 Standards (lyophilized) 2 vials Pre-coated canine IL-10 Microplate (12 strips by 8 wells) 1 unit Stop Solution 1 x 8ml TMB One-Step Substrate Reagent 1 x 12ml -
Research areas
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Function
Inhibits the synthesis of a number of cytokines, including IFN-gamma, IL-2, IL-3, TNF and GM-CSF produced by activated macrophages and by helper T-cells. -
Tissue specificity
Produced by a variety of cell lines, including T-cells, macrophages, mast cells and other cell types. -
Sequence similarities
Belongs to the IL-10 family. -
Cellular localization
Secreted. - Information by UniProt
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Alternative names
- CSIF
- Cytokine synthesis inhibitory factor
- GVHDS
see all
Images
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Typical standard curve
Data provided for demonstration purposes only. A new standard curve must be generated for each assay performed.
