Calcein Blue AM acetate (ab275490)
Key features and details
- Cell-permeant, blue fluorescent viability probe.
- CAS Number: 168482-84-6
- Purity: > 98%
Soluble in DMSO.
- Form / State: Solid
- Source: Synthetic
Overview
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Product name
Calcein Blue AM acetate -
Description
Cell-permeant, blue fluorescent viability probe. -
Biological description
Blue fluorescent cell viability probe which won't overlap with green fluorescence from other probes. Penetrates live cell membranes and is virtually non-fluorescent. Upon ubiquitous esterase enzyme activity it produces a bright blue fluorescent that is trapped in live cells - convenient for measurement by epifluorescence light microscopy or flow cytometry. Useful to measure the total number of live versus dead cells - dye leaks out quickly due to the compromised membrane in dead cells resulting in no staining.
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Purity
> 98% -
CAS Number
168482-84-6 -
Chemical structure
Properties
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Chemical name
N-[2-[(Acetyloxy)methoxy]-2-oxoethyl]-N-[(7-hydroxy-4-methyl-2- oxo-2H-1-benzopyran-8-yl)methyl]- glycine, (acetyloxy)methyl ester -
Molecular weight
507.44 -
Molecular formula
C23H25NO12 -
PubChem identifier
71774480 -
Storage instructions
Shipped at 4°C. Store at -20°C. Store In the Dark. Store under desiccating conditions. -
Solubility overview
Soluble in DMSO.
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Handling
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
Refer to SDS for further information.
Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.
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SMILES
CC1=CC(=O)OC2=C1C=CC(=C2CN(CC(=O)OCOC(=O)C)CC(=O)OCOC(=O)C)O -
Source
Synthetic
Images
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Chemical Structure - Calcein Blue AM acetate (ab275490)
Chemical structure of Calcein Blue AM acetate, 168482-84-6