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Bovine VEGF-A ELISA Kit (ab273194)

Key features and details

  • Sensitivity: 0.12 ng/ml
  • Range: 0.12 ng/ml - 30 ng/ml
  • Sample type: Cell culture supernatant, Plasma, Serum
  • Detection method: Colorimetric
  • Assay type: Sandwich (quantitative)
  • Reacts with: Cow

Overview

  • Product name

    Bovine VEGF-A ELISA Kit
    See all VEGFA kits

  • Detection method

    Colorimetric

  • Precision

    Intra-assay
    Sample n Mean SD CV%
    Overall
    Inter-assay
    Sample n Mean SD CV%
    Overall

  • Sample type

    Cell culture supernatant, Serum, Plasma

  • Assay type

    Sandwich (quantitative)

  • Sensitivity

    0.12 ng/ml

  • Range

    0.12 ng/ml - 30 ng/ml

  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Serum 71.91 68% - 80%
    Plasma 70.95 68% - 77%
    Cell culture media 87.89 79% - 102%

  • Assay duration

    Multiple steps standard assay

  • Species reactivity

    Reacts with: Cow

  • Product overview

    Bovine VEGF-A ELISA Kit (ab273194) is an in-vitro enzyme-linked immunosorbent assay for the quantitative measurement of Bovine VEGF-A in serum (bovine VEGF-A concentration is low in normal serum/plasma, and may not be detectable in this assay), plasma and cell culture supernatants.


    This assay employs an antibody specific for Bovine VEGF-A coated on a 96-well plate. Standards and samples are pipetted into the wells and VEGF-A present in a sample is bound to the wells by the immobilized antibody. The wells are washed, and biotinylated anti-Bovine VEGF-A antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of VEGF-A bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • Tested applications

    Suitable for: Sandwich ELISAmore details

  • Platform

    Pre-coated microplate (12 x 8 well strips)

Properties

  • Storage instructions

    Store at -20°C. Please refer to protocols.
  • Components 1 x 96 tests
    20X Wash Buffer 1 x 25ml
    250X HRP-Streptavidin Concentrate 1 x 200µl
    5X Assay Diluent 1 x 15ml
    Anti-Bovine VEGF-A coated Microplate (12 x 8 wells) 1 unit
    Biotinylated Anti-Bovine VEGF-A Antibody 2 vials
    Bovine VEGF-A Standard (Lyophilized) 2 vials
    Stop Solution 1 x 8ml
    TMB Substrate Solution 1 x 12ml

  • Research areas

  • Function

    Growth factor active in angiogenesis, vasculogenesis and endothelial cell growth. Induces endothelial cell proliferation, promotes cell migration, inhibits apoptosis and induces permeabilization of blood vessels. Binds to the FLT1/VEGFR1 and KDR/VEGFR2 receptors, heparan sulfate and heparin. NRP1/Neuropilin-1 binds isoforms VEGF-165 and VEGF-145. Isoform VEGF165B binds to KDR but does not activate downstream signaling pathways, does not activate angiogenesis and inhibits tumor growth.

  • Tissue specificity

    Isoform VEGF189, isoform VEGF165 and isoform VEGF121 are widely expressed. Isoform VEGF206 and isoform VEGF145 are not widely expressed.

  • Involvement in disease

    Defects in VEGFA are a cause of susceptibility to microvascular complications of diabetes type 1 (MVCD1) [MIM:603933]. These are pathological conditions that develop in numerous tissues and organs as a consequence of diabetes mellitus. They include diabetic retinopathy, diabetic nephropathy leading to end-stage renal disease, and diabetic neuropathy. Diabetic retinopathy remains the major cause of new-onset blindness among diabetic adults. It is characterized by vascular permeability and increased tissue ischemia and angiogenesis.

  • Sequence similarities

    Belongs to the PDGF/VEGF growth factor family.

  • Cellular localization

    Secreted. VEGF121 is acidic and freely secreted. VEGF165 is more basic, has heparin-binding properties and, although a signicant proportion remains cell-associated, most is freely secreted. VEGF189 is very basic, it is cell-associated after secretion and is bound avidly by heparin and the extracellular matrix, although it may be released as a soluble form by heparin, heparinase or plasmin.
  • Information by UniProt

  • Alternative names

    • Folliculostellate cell-derived growth factor
    • Glioma-derived endothelial cell mitogen
    • MGC70609
    • MVCD1
    • vascular endothelial growth factor
    • Vascular endothelial growth factor A
    • vascular endothelial growth factor A121
    • vascular endothelial growth factor A165
    • Vascular permeability factor
    • Vegf
    • VEGF A
    • VEGF-A
    • VEGF120
    • Vegfa
    • VEGFA_HUMAN
    • VPF
    see all

  • Database links

    Images

    • Example standard curve
      Example standard curve

      This standard curve is for demonstration only. A standard curve must be run with each assay.

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