Bovine Interferon gamma ELISA Kit (IFNG) (ab193681)
Key features and details
- Sensitivity: 0.11 ng/ml
- Range: 0.11 ng/ml - 30 ng/ml
- Sample type: Cell culture supernatant, Plasma
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Cow
Overview
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Product name
Bovine Interferon gamma ELISA Kit (IFNG)
See all Interferon gamma kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Overall Inter-assay Sample n Mean SD CV% Overall -
Sample type
Cell culture supernatant, Plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
0.11 ng/ml -
Range
0.11 ng/ml - 30 ng/ml -
Recovery
Sample specific recovery Sample type Average % Range Plasma = 96.18 87% - 104% Cell culture media = 113.2 98% - 120% -
Species reactivity
Reacts with: Cow -
Product overview
Abcam’s IFN gamma bovine ELISA Kit (ab193681) is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of bovine IFN gamma in serum, plasmas and cell culture supernatant. Plasma sample should be collected using EDTA or citrate as an anticoagulant. Heparin is not recommended for use in this assay.
This assay employs an antibody specific for bovine IFN gamma coated on a 96-well plate. Standards and samples are pipetted into the wells and the immobilized antibody captures IFN gamma present in the samples. The wells are washed and biotinylated anti-bovine IFN gamma antibody is added. After washing away any unbound biotinylated antibody, an HRP-conjugated streptavidin is pipetted to the wells. After incubation, the wells are again washed, followed by the addition of a TMB substrate solution to the wells. Color will develop in proportion to the amount of IFN gamma bound in each well. Addition of the Stop Solution will change the color from blue to yellow, and the intensity of the color is measured at 450 nm.
We have not been able to detect endogenous Bovine Interferon gamma in normal serum with ab193681, only in serum spiked with Bovine Interferon gamma.
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Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 1 x 96 tests 20X Wash Buffer Concentrate 1 x 25ml 5X Assay Diluent 1 x 15ml HRP-Streptavidin Concentrate (Item G) 1 x 200µl Biotinylated Bovine IFN-gamma Detection Antibody (lyophilized) 1 x 2 vials Bovine IFN-gamma Standards (lyophilized) 1 x 2 vials Pre Coated Bovine IFN-gamma Microplate (12 strips x 8 wells) 1 unit Stop Solution 1 x 8ml TMB One-Step Substrate Reagent 1 x 12ml -
Research areas
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Function
Produced by lymphocytes activated by specific antigens or mitogens. IFN-gamma, in addition to having antiviral activity, has important immunoregulatory functions. It is a potent activator of macrophages, it has antiproliferative effects on transformed cells and it can potentiate the antiviral and antitumor effects of the type I interferons. -
Tissue specificity
Released primarily from activated T lymphocytes. -
Involvement in disease
In Caucasians, genetic variation in IFNG is associated with the risk of aplastic anemia (AA) [MIM:609135]. AA is a rare disease in which the reduction of the circulating blood cells results from damage to the stem cell pool in bone marrow. In most patients, the stem cell lesion is caused by an autoimmune attack. T-lymphocytes, activated by an endogenous or exogenous, and most often unknown antigenic stimulus, secrete cytokines, including IFN-gamma, which would in turn be able to suppress hematopoiesis. -
Sequence similarities
Belongs to the type II (or gamma) interferon family. -
Post-translational
modificationsProteolytic processing produces C-terminal heterogeneity, with proteins ending alternatively at Gly-150, Met-157 or Gly-161. -
Cellular localization
Secreted. - Information by UniProt
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Alternative names
- IF 1
- IFG
- IFI
see all -
Database links
- Entrez Gene: 281237 Cow
- SwissProt: P07353 Cow
Images
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Standard Curve
Example of typical bovine IFN gamma standard curve using Assay Diluent (used for serum/plasma standards).