Bovine IgG ELISA kit - 45 minutes (ab273152)
Key features and details
- Range: 10 ng/ml - 2000 ng/ml
- Detection method: Colorimetric
- Assay type: Quantitative
Overview
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Product name
Bovine IgG ELISA kit - 45 minutes
See all IgG kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% 1/100 32 38µg/ml 6.5 % 1/200 32 44.17µg/ml 7.6 % 1/400 32 41.55µg/ml 10.8 % Inter-assay Sample n Mean SD CV% A 1/16 14 7.3µg/ml 5.87 % B 1/128 14 7.7µg/ml 5.89 % Bovine 1/100 16 1.2µg/ml 4.3 % -
Assay type
Quantitative -
Range
10 ng/ml - 2000 ng/ml -
Assay duration
One step assay -
Product overview
The Bovine Immunoglobulin G ELISA Kit (ab273152) provides a rapid and easy method (one step ELISA) for the quantitative determination of bovine IgGs in cell culture supernatants and serums (CS, FCS, NBCS...) and contaminating bovine IgGs in batches of purified antibodies produced in vitro. The kit includes ready-to-use reagents necessary to analyze 89 samples in less than 45 minutes.
A polyclonal antibody specific to bovine IgG is pre-coated onto microwells. Samples and standards are pipetted into microwells and bovine IgG present in the sample are bound by the capture antibody. Then, an HRP (horseradish peroxidase) conjugated anti-bovine IgG (H+L) antibody is pipetted and incubated simultaneously with samples. After washing microwells in order to remove any non specific binding, the ready to use substrate solution (TMB) is added to microwells and color develops proportionally to the amount of bovine IgG in the sample. Color development is then stopped by addition of stop solution. Absorbance is measured at 450 nm.
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Tested applications
Suitable for: ELISAmore details -
Platform
Microplate
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10 x 96 tests Bovine IgG standard 0.0 ng/ml 1 x 0.3ml 1 x 1ml Bovine IgG standard 1000 ng/ml 1 x 0.3ml 1 x 1ml Bovine IgG standard 125 ng/ml 1 x 0.3ml 1 x 1ml Bovine IgG standard 2000 ng/ml 1 x 0.3ml 1 x 1ml Bovine IgG standard 250 ng/ml 1 x 0.3ml 1 x 1ml Bovine IgG standard 31 ng/ml 1 x 0.3ml 1 x 1ml Bovine IgG standard 500 ng/ml 1 x 0.3ml 1 x 1ml Detection antibody 1 x 12ml 1 x 120ml Pre-coated microwells strips 1 x 96 tests 10 x 96 tests Sample Diluent 1 x 30ml 1 x 500ml Stop solution 1 x 12ml 1 x 120ml TMB Substrate 1 x 12ml 1 x 120ml -
Research areas
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Cellular localization
Secreted -
Alternative names
- Ig gamma 1 chain C region
- Ig gamma 2 chain C region
- Ig gamma 3 chain C region
see all
Images
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Bovine Immunoglobulin G Quantification Kit Standard curve
Example standard curve.
