Bovine CXCL9 ELISA Kit (ab273200)
Key features and details
- Sensitivity: 32 pg/ml
- Range: 32 ng/ml - 8000 ng/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Cow
Overview
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Product name
Bovine CXCL9 ELISA Kit
See all CXCL9 kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Overall Inter-assay Sample n Mean SD CV% Overall -
Sample type
Cell culture supernatant, Serum, Plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
32 pg/ml -
Range
32 ng/ml - 8000 ng/ml -
Recovery
Sample specific recovery Sample type Average % Range Serum 105.8 90% - 120% Plasma 94.17 70% - 135% Cell culture media 75.73 69% - 93% -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Cow -
Product overview
Bovine CXCL9 ELISA Kit (ab273200) is an in-vitro enzyme-linked immunosorbent assay for the quantitative measurement of bovine CXCL9 in serum, plasma and cell culture supernatants.
This assay employs an antibody specific for bovine CXCL9 coated on a 96-well plate. Standards and samples are pipetted into the wells and CXCL9 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-bovine CXCL9 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of CXCL9 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
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Tested applications
Suitable for: ELISAmore details -
Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 1 x 96 tests 20X Wash Buffer 1 x 25ml 5X Assay Diluent 1 x 15ml 800X HRP-Streptavidin Concentrate 1 x 200µl Anti-Bovine CXCL9 coated Microplate (12 x 8 wells) 1 unit Biotinylated Anti-Bovine CXCL9 Antibody 2 vials Bovine CXCL9 Standard (Lyophilized) 2 vials Stop Solution 1 x 8ml TMB Substrate Solution 1 x 12ml -
Research areas
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Function
Cytokine that affects the growth, movement, or activation state of cells that participate in immune and inflammatory response. Chemotactic for activated T-cells. Binds to CXCR3. -
Sequence similarities
Belongs to the intercrine alpha (chemokine CxC) family. -
Cellular localization
Secreted. - Information by UniProt
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Alternative names
- C-X-C motif chemokine 9
- chemokine (C-X-C motif) ligand 9
- CMK
see all -
Database links
- Entrez Gene: 513990 Cow
- SwissProt: A9QWP9 Cow
Images
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Example data
This standard curve is for demonstration only. A standard curve must be run with each assay.
