Bisulfite Conversion Kit - Whole Cell (ab117126)
Key features and details
- Assay time: 3 hr
- Sample type: Adherent cells, Saliva, Suspension cells, Tissue, Urine
Overview
-
Product name
Bisulfite Conversion Kit - Whole Cell
See all DNA modification kits -
Sample type
Saliva, Urine, Tissue, Adherent cells, Suspension cells -
Assay time
3h 00m -
Species reactivity
Reacts with: Human
Predicted to work with: Mammals
-
Product overview
DNA methylation is a covalent modification of the cytosine ring at the 5' position of a CpG dinucleotide which leads to epigenetic inactivation of genes when found in 5'-CpG-3'dinucleotides within promoters or in the first exon of genes. It is well demonstrated that DNA methylation plays an important role in the regulation of gene expression, tumorigenesis, and other genetic and epigenetic diseases.
Most methods for DNA methylation detection require a prior bisulfite-based DNA modification. Bisulfite-based DNA modification is used to discrimate between cytosine and methylated cytosine, in which DNA is treated with bisulfite salt to convert cytosine residues to uracil in a single-stranded DNA, while methylated cytosine remains the same.
Bisulfite Modification Kit - Whole Cell (ab117126) allows the user to perform DNA methylation analysis and modify DNA directly from cells or tissues within only 3 hours. ab117126 is specifically designed for DNA methylation research using minute amounts of starting materials including cells cultured in 96-well/384-well plates, tissue section samples, microdissection samples, tissue biopsy and early embryonic cells/oocytes.
Modified DNA is eluted and suitable for real time MS-PCR as well as for all techniques currently used for the analysis of DNA methylation; including conventional MS-PCR, bisulfite sequencing, pyrosequencing, and methylation microarray.
Not sure if this is the right kit for you? Check out our bisulfite modification guide for more information.
-
Notes
Properties
-
Storage instructions
Please refer to protocols. -
Components 40 tests 80 tests Balance Buffer 1 x 0.4ml 1 x 0.8ml Cell Collection Buffer 1 x 1ml 1 x 2ml Digestion Powder 1 vial 1 vial Digestion Solution 1 x 0.1ml 1 x 0.2ml DNA Binding Buffer 1 x 14ml 1 x 28ml DNA Modification Buffer 1 x 5ml 1 x 10ml DNA Modification Powder 4 vials 8 vials F-Collection Tube 1 x 40 units 1 x 80 units F-Spin Column 1 x 40 units 1 x 80 units Modified DNA Elution 1 x 1ml 1 x 2ml -
Research areas
Images
-
Functional Studies - Bisulfite Modification Kit - Whole Cell (ab117126)MCF-7 cells were modified using ab117126. 10 µl of modified DNA were eluted and 2 µl of elution were used in real time PCR. A pair of primers and a probe designed to amplify both methylated and unmethylated alleles of ß-actin was used.
