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Neuroscience Neurology process Growth and Development Neurotrophins

Biotin Anti-NGF antibody (ab271277)

Biotin Anti-NGF antibody (ab271277)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Biotin Rabbit polyclonal to NGF
  • Suitable for: WB, ELISA
  • Reacts with: Recombinant fragment
  • Conjugation: Biotin
  • Isotype: IgG

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Overview

  • Product name

    Biotin Anti-NGF antibody
    See all NGF primary antibodies
  • Description

    Biotin Rabbit polyclonal to NGF
  • Host species

    Rabbit
  • Conjugation

    Biotin
  • Tested Applications & Species

    Application Species
    ELISA
    Recombinant fragment
    WB
    Recombinant fragment
    See all applications and species data
  • Immunogen

    Recombinant full length protein corresponding to Human NGF aa 100 to the C-terminus. Mature chain. E. coli derived.
    Database link: P01138

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive control

    • WB: Recombinant human NGF.

Properties

  • Form

    Lyophilized:Reconstitute in sterile PBS with 0.1% BSA to 0.1 - 1.0 mg/ml.
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. Store In the Dark.
  • Storage buffer

    Constituents: PBS, 0.1% BSA
  • Concentration information loading...
  • Purity

    Affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Neuroscience
    • Neurology process
    • Growth and Development
    • Neurotrophins
    • Neuroscience
    • Neurology process
    • Neuroregeneration
    • Neuroregeneration

Images

  • Western blot - Biotin Anti-NGF antibody (ab271277)
    Western blot - Biotin Anti-NGF antibody (ab271277)

    Lane 1: Marker.
    Lane 2: Recombinant hNGF at 250ng/lane.
    Lane 3: Recombinant hNGF at 125ng/lane.
    Lane 4: Recombinant hNGF at 62.5ng/lane.
    Lane 5: Recombinant hNGF at 31.25ng/lane.
    Lane 6: Recombinant hNGF at 15.625ng/lane.
    Lane 7: Recombinant hNGF at 7.8ng/lane.
    Lane 8: Recombinant hNGF at 3.9ng/lane.
    Lane 9: Recombinant hNGF at 1.95ng/lane.
    Lane 10: Recombinant hNGF at 0.975ng/lane.
    Lane 11: Recombinant hNGF at 0.4875ng/lane.
    Lane 12: Recombinant hNGF at 0.24ng/lane.

  • ELISA - Biotin Anti-NGF antibody (ab271277)
    ELISA - Biotin Anti-NGF antibody (ab271277)
    Human NGF was detected by sandwich ELISA (using 100 µl/well) using a concentration of 0.25-1.0 µg/ml of ab271277. This antibody in conjunction with an appropriate capture antibody, allows the detection of at least XXXX of recombinant NGF protein.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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