Biotin Anti-AKT1 (phospho T308) antibody [18F3.H11] (ab272114)
![Biotin Anti-AKT1 (phospho T308) antibody [18F3.H11] (ab272114)](https://www.abcam.com/ps/products/272/ab272114/Images/ab272114-1-AKTpT308BiotinConjugatedMonoclonalAntibody1IHC.jpg "Biotin Anti-AKT1 (phospho T308) antibody [18F3.H11] (ab272114)")
Key features and details
- Biotin Mouse monoclonal [18F3.H11] to AKT1 (phospho T308)
- Suitable for: IHC-P, WB, ELISA
- Reacts with: Human, Recombinant fragment
- Conjugation: Biotin
- Isotype: IgG1
Overview
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Product name
Biotin Anti-AKT1 (phospho T308) antibody [18F3.H11]
See all AKT1 primary antibodies -
Description
Biotin Mouse monoclonal [18F3.H11] to AKT1 (phospho T308) -
Host species
Mouse -
Conjugation
Biotin -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P HumanWB Recombinant fragment
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Immunogen
Synthetic peptide within Human AKT1 (phospho T308). The exact sequence is proprietary. Synthetic peptide corresponding to residues surrounding T308 of human AKT1 protein.
Database link: P31749 -
Positive control
- IHC-P: Human prostate tissue. WB: Active recombinant AKT1 GST tagged protein.
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General notes
10-20 moles Biotin per mole of IgG1
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. Store In the Dark. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride, 1% BSA
Buffer lyophilised from -
Concentration information loading... -
Purity
Protein A purified -
Purification notes
Purified from tissue culture supernatant. -
Clonality
Monoclonal -
Clone number
18F3.H11 -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Biotin Anti-AKT1 (phospho T308) antibody [18F3.H11] (ab272114)
Formalin-fixed, paraffin embedded human prostate tissue stained for AKT1 using ab272114 at 20 µg/mL in immunohistochemical analysis.
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![Western blot - Biotin Anti-AKT1 (phospho T308) antibody [18F3.H11] (ab272114)](https://www.abcam.com/ps/products/272/ab272114/Images/ab272114-3-AKTpT308BiotinConjugatedMonoclonalAntibody2WB.jpg)
Western blot - Biotin Anti-AKT1 (phospho T308) antibody [18F3.H11] (ab272114)All lanes : Biotin Anti-AKT1 (phospho T308) antibody [18F3.H11] (ab272114) at 1/1000 dilution
Lane 1 : Active recombinant AKT1 GST-tagged protein, 25 ng
Lane 2 : Inactive recombinant AKT1 GST-tagged protein, 25 ng
Secondary
All lanes : Streptavidin-HRP at 1/40000 dilution
Predicted band size: 79 kDa
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![ELISA - Biotin Anti-AKT1 (phospho T308) antibody [18F3.H11] (ab272114)](https://www.abcam.com/ps/products/272/ab272114/Images/ab272114-2-AKTpT308BiotinConjugatedMonoclonalAntibody3ELISA.jpg)
ELISA - Biotin Anti-AKT1 (phospho T308) antibody [18F3.H11] (ab272114)ELISA of AKT1 phospho T308 (red) and AKT non-phospho T308 (blue) at 0.1 µg/well using ab272114 at 5 µg/mL and 3-fold serial diluted, followed by Streptavidin-Peroxidase at 1/10,000. Substrate: TMB.