Beta-Glucuronidase (GUS) Reporter Gene Activity Detection Kit (ab253372)
Key features and details
- Detection method: Fluorescent
Overview
-
Product name
Beta-Glucuronidase (GUS) Reporter Gene Activity Detection Kit -
Detection method
Fluorescent -
Product overview
Allows for quantitative measurement of GUS enzyme activity in transformed plants through use of a fluorogenic substrate.
The β-glucuronidase (GUS) enzyme from E. coli (EC 3.2.1.31) has been well documented to provide desirable characteristics as a marker gene in transformed plants. The GUS reporter gene system has many advantages including stable expression of E. coli GUS enzyme, no interference with normal plant metabolism, and low intrinsic GUS activity in higher plants. The enzyme is also capable of tolerating amino-terminal additions, making it useful for study of plant organelle transport. Various β-glucuronic acid substrates are available for detection of GUS expression, all of which contain the sugar D-glucopyranosiduronic acid attached by glycosidic linkage to a hydroxyl group of a chromogenic, fluorogenic, or other detectable molecule. The most widely used fluorogenic substrate for detection of β-glucuronidase activity in vitro is 4-methylumbelliferyl β-D-glucuronide (4-MUG). Upon hydrolysis by GUS, the fluorochrome 4-methylumbelliferone (7-hydroxy-4-methyl coumarin) is produced along with sugar glucuronic acid. It is used in this fluorescent activity detection test.
This kit is sold by Marker Gene under product code M0877.
-
Notes
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Properties
-
Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 units 4-MU Calibration Stock Solution 1 x 5ml Carbonate Stop Buffer 1 x 25ml GUS Assay Buffer 1 x 5ml GUS Extraction Buffer 1 x 25ml -
Research areas
-
Relevance
Reporter genes are widely used for studying the expression of foreign genes in transformed plants tissues. Using appropriate promoter-reporter gene constructs, this technique allows an independent verification of the transformed status of tissues growing on media containing selective antibiotics or herbicides. In addition, it serves as a principal means to follow gene transfer and monitor genetic transformation of plant species. Encoded by the E. coli GUS gene (also referred to as uidA), GUS protein is a hydrolase that catalyses the cleavage of a variety of beta-glucuronide derivatives available for colorimetric, fluorimetric and histochemical assays. Several features make the gus gene superior as a reporter gene for plant studies and in the production of genetically engineered crops. -
Cellular localization
Cytoplasmic -
Alternative names
- Beta D glucuronidase
- Beta glucuronidase
- gurA
- UidA
