beta-Glucosidase Assay Kit (ab272521)
Key features and details
- Assay type: Quantitative
- Detection method: Colorimetric
- Platform: Microplate
Overview
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Product name
beta-Glucosidase Assay Kit
See all beta-Glucosidase kits -
Detection method
Colorimetric -
Assay type
Quantitative -
Range
2 mU/ml - 250 mU/ml -
Product overview
Beta-Glucosidase Assay Kit (ab272521) is a simple, direct and automation-ready procedure for measuring β-glucosidase activity. beta-Glucosidase Assay Kit is designed to measure β-glucosidase activity directly in biological samples without pretreatment. The improved method utilizes p-nitrophenyl-β-D-glucopyranoside that is hydrolyzed specifically by β-glucosidase into a yellow colored product (maximal absorbance at 405nm). The rate of the reaction is directly proportional to the enzyme activity.
High sensitivity and wide linear range. Use 20 μL sample. The detection limit is 2 U/L, linear up to 250 U/L.
Homogeneous and simple procedure. Simple "mix-and-measure" procedure allows reliable quantitation of β-glucosidase activity within 20 minutes.
Robust and amenable to HTS. All reagents are compatible with high-throughput liquid handling instruments.
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Tested applications
Suitable for: Functional Studiesmore details -
Platform
Microplate
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 100 tests β-NPG Substrate 1 x 1ml Assay Buffer 1 x 24ml Calibrator 1 x 10ml
Images
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Standard Curve
Typical standard curve – data provided for demonstration purposes only. A new standard curve must be generated for each assay performed.
Example of beta-Glucosidase standard curve.
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Progress CurvesExample of beta-Glucosidase reaction progress curves.