BaseMuncher Endonuclease (ab270049)
Overview
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Product name
BaseMuncher Endonuclease -
General notes
Endonuclease enzyme BaseMuncher is a non-specific endonuclease that is used for the removal of nucleic acid from protein samples. Our ultrapure endonuclease hydrolyzes both single stranded and double stranded, along with circular and linear nucleic acids (DNA and RNA) to 5-phosphorylated oligonucleotides of 1-4 bases in length.
Recombinantly produced in E. coli using the Benzonase gene in a proprietary process, BaseMuncher is a highly purified homodimer of 27kDa subunits that have exceptionally high specific activity and is completely free of protease activity. BaseMuncher is ideal for reducing viscosity during protein purification and sample preparation.
One Unit of BaseMuncher converts 1.0 OD260 of salmon sperm DNA into acid soluble nucleotides in 30 minutes at 37°C in a reaction buffer of 50mM Tris-HCl, pH 8.0 and 1mM MgCl2. This corresponds to complete digestion of 50g of salmon sperm DNA into oligonucleotides.
Features & Benefits:
Exceptionally high endonuclease specificity for DNA and RNA
Ultrapure to >99%
Free of protease activity
High purity and high concentration grade
Easy protein purification –Reduced viscosity during sample preparation
100x greater activity than DNAase
Cheaper than Benzonase; but the same quality and efficiency.
General notes:
This product is manufactured by Expedeon, an Abcam company. Expedeon product code BM0025 was previously called BaseMuncher Endonuclease >99% Ultrapure - 25,000 Units (1 x 100 µL) and is the same as the 25,000 unit size of this product. Expedeon product code BM0100 was previously called BaseMuncher Endonuclease >99% Ultrapure - 100,000 Units (4 x 100 µL) and is is the same as the 100,000 unit size.
Properties
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Form
Liquid -
Storage instructions
Shipped at room temperature. Store at -20°C. -
Storage buffer
Constituents: 50% Glycerol (glycerin, glycerine), 0.1% Magnesium chloride hexahydrate, 0.6% Tris, 0.3% Sodium chloride, 45% Water, 0.025% Enzyme -
Concentration information loading...
Images
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RIP - BaseMuncher Endonuclease (ab270049)
BaseMuncher Endonuclease is purified by a proprietary process to be >99% pure by SDS-PAGE.
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Functional Studies - BaseMuncher Endonuclease (ab270049)50 mg of salmon sperm DNA was incubated with the indicated units of BaseMuncher Endonuclease and another brand of nuclease at 37°C for 30 mins in a buffer containing 50 mM Tris-HCl; pH 8.0 and 1 mM MgCl2.
DNA digestion was monitored by agarose gel.
