Arf1 Activation Assay Kit (ab211170)
Key features and details
- Sample type: Adherent cells, Suspension cells, Tissue
Overview
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Product name
Arf1 Activation Assay Kit
See all ARF1 kits -
Sample type
Tissue, Adherent cells, Suspension cells -
Species reactivity
Reacts with: Mouse, Rat, Human -
Product overview
Arf1 Activation Assay Kit (ab211170) uses GGA3 PBD Agarose beads to selectively isolate and pull-down the active form of Arf from purified samples or endogenous lysates. Subsequently, the precipitated GTP-Arf is detected by western blot analysis using an anti-Arf1 antibody, which detects the human, mouse and rat protein.
Features: 1) non radioactive assay format; 2) fast results: 1 hour assay plus electrophoresis/blotting time; 3) includes Cdc42 positive control; 4) pink colored agarose beads for easy identification during washing and aspiration steps.
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Notes
Small GTP-binding proteins (or GTPases) are a family of proteins that serve as molecular regulators in signaling transduction pathways.
Arf1 (ADP-ribosylation factor 1) is a 21 kDa protein, member of ARF gene family, which constitutes part of the Ras superfamily. The Arf proteis are categorized as class I (Arf1, Arf2, Arf3), class II (Arf4 and Arf5) and class III (Arf6), and they all share a common gene organization. Arf1 regulates a variety of biological response pathways that include vesicle and protein trafficking, organelle structure, and endocytosis. Like other small GTPases, Arf1 cycles between an inactive GDP-bound form and an active GTP-bound form: in its active (GTP-bound) state, Arf1 binds specifically to the protein-binding domain (PBD) of GGA3 to control downstream signaling cascades.
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 20 tests 100X GDP 1 x 50µl 100X GTPyS 1 x 50µl 5X Assay/Lysis Buffer 1 x 30ml ab211180 - GGA3 PBD Agarose Beads 1 x 800µl anti-Arfl, Mouse monoclonal antibody 1 x 40µl GTPase immunoblot Positive Control 1 x 100µl -
Research areas
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Function
GTP-binding protein that functions as an allosteric activator of the cholera toxin catalytic subunit, an ADP-ribosyltransferase. Involved in protein trafficking among different compartments. Modulates vesicle budding and uncoating within the Golgi complex. Deactivation induces the redistribution of the entire Golgi complex to the endoplasmic reticulum, suggesting a crucial role in protein trafficking. In its GTP-bound form, its triggers the association with coat proteins with the Golgi membrane. The hydrolysis of ARF1-bound GTP, which is mediated by ARFGAPs proteins, is required for dissociation of coat proteins from Golgi membranes and vesicles. -
Sequence similarities
Belongs to the small GTPase superfamily. Arf family. -
Cellular localization
Golgi apparatus. Cytoplasm > perinuclear region. - Information by UniProt
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Alternative names
- ADP Ribosylation Factor 1
- ADP-ribosylation factor 1
- ARF 1
see all -
Database links
- Entrez Gene: 375 Human
- Entrez Gene: 11840 Mouse
- Entrez Gene: 64310 Rat
- Omim: 103180 Human
- SwissProt: P84077 Human
- SwissProt: P84078 Mouse
- SwissProt: P84079 Rat
- Unigene: 286221 Human
see all
Images
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Arf1 Activation Assay Kit (ab211170)
Arf1 activation assay. Lane 1: HeLa cell lysate loaded with GDP and incubated with GGA3 PBD Agarose beads. Lane 2: HeLa cell lysate loaded with GTPγS and incubated with GGA3 PBD Agarose beads.
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Arf1 Activation Assay Kit (ab211170)Arf1 positive control (293 cell lysate at 0.5 mg/mL) detected by immunoblot.