APC Anti-CD4 antibody [RM4-5] (ab210347)
![APC Anti-CD4 antibody [RM4-5] (ab210347)](https://www.abcam.com/ps/products/210/ab210347/Images/ab210347-256507-anti-cd4-antibody-rm4-5-allophycocyanin-flow-cytometry.jpg "APC Anti-CD4 antibody [RM4-5] (ab210347)")
Key features and details
- APC Rat monoclonal [RM4-5] to CD4
- Suitable for: Flow Cyt
- Reacts with: Mouse
- Conjugation: APC. Ex: 645nm, Em: 660nm
- Isotype: IgG2a
Overview
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Product name
APC Anti-CD4 antibody [RM4-5]
See all CD4 primary antibodies -
Description
APC Rat monoclonal [RM4-5] to CD4 -
Host species
Rat -
Conjugation
APC. Ex: 645nm, Em: 660nm -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt Rat
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Positive control
- C57Bl/6 splenocytes.
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General notes
The RM4-5 antibody is widely used as a phenotypic marker for CD4 expression. If used together, the RM4-5 antibody and an alternative antibody, Anti-Mouse CD4 clone GK1.5, will “compete” for binding, i.e. RM4-5 antibody is able to block GK1.5 antibody binding to cells. In contrast, RM4-5 antibody does not block the binding of Anti-Mouse CD4 clone RM4-4 to cells.
The purified antibody was conjugated under optimal conditions, with unreacted dye removed from the preparation.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. Store In the Dark. Store undiluted. -
Storage buffer
pH: 7.20
Preservative: 0.09% Sodium azide
Constituents: 0.12% Monobasic dihydrogen sodium phosphate, 0.87% Sodium chloride, 0.1% Gelatin -
Concentration information loading... -
Purity
Protein A purified -
Purification notes
ab210347 was purified from tissue culture supernatant via affinity chromatography. The purified antibody was conjugated under optimal conditions, with unreacted dye removed from the preparation. -
Clonality
Monoclonal -
Clone number
RM4-5 -
Isotype
IgG2a -
Light chain type
kappa -
Research areas
Images
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Flow Cytometry - APC Anti-CD4 antibody [RM4-5] (ab210347)
Flow cytometric analysis of C57Bl/6 splenocytes labeling CD4 with 0.125 µg ab210347 (solid line), compared with 0.125 µg APC Rat IgG2a isotype control (dashed line).
