The Antibody Concentration and Clean Up Kit for Latex & Europium kit (ab269965) removes small molecule buffer components from the antibody or protein prior to use in the conjugation reaction. Some buffer components interfere with the conjugation reaction of the Latex & Europium conjugation kits, reducing or entirely preventing the conjugation to the Latex nanoparticles.

This product is manufactured by Expedeon, an Abcam company. It was previously called Antibody Concentration & Clean Up Kit for Latex and Europium. Expedeon product code 1020-0040 is the same as the 4 unit size of this product.

This kit utilizes a simple spin column to clean up the antibody by buffer exchanging to remove the unwanted buffer components. The antibody is quickly and easily concentrated and then diluted with one of the Conjugation Kit Reaction Buffers for Latex and Europium. This step is repeated several times. This exchanges the antibody into a buffer perfect for the conjugation reaction and reduces the concentration of the initial buffer by several orders of magnitude so interference does not occur.

There are two Conjugation Kit Reaction Buffers, A and B, both of which are provided in this kit. One Spin Cartridge/Collecting Tube Assembly can clean up an antibody into one of the two buffers. To clean up the antibody into both Reaction Buffers, two Spin Cartridge/Collecting Tube Assemblies must be used.

The optimal buffer for conjugation varies for different antibodies, which is why both are provided. An antibody cleaned up into 1x Reaction Buffer A, but conjugated in 1x Reaction Buffer B (and vice versa), will have a conjugation efficiency of 50 – 100 % compared to both cleaning up and conjugating in the optimal buffer. For optimal conjugation efficiency we therefore recommend determining the optimal 1x Reaction Buffer then ensuring antibody for future conjugations is cleaned up into the optimal buffer. For quick scouting or ‘proof of principle’ experiments this may not be necessary.

If needed the Antibody Concentration & Clean Up Kit for Latex and Europium can also be used to concentrate the antibody by recovering the antibody in a smaller volume than the volume initially added to the Spin Cartridge.

ΔNote: This kit cannot remove proteins such as BSA and Gelatin from antibody buffer.