Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-ZNHIT1 antibody [EPR20922-47] (ab238125)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR20922-47] to ZNHIT1
  • Suitable for: WB, IP
  • Knockout validated
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-ZNHIT1 antibody [EPR20922-47]
    See all ZNHIT1 primary antibodies

  • Description

    Rabbit monoclonal [EPR20922-47] to ZNHIT1

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IP
    Mouse
    Human
    WB
    Mouse
    Rat
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: HeLa, MEF, C2C12, HEK-293T and PC12 whole cell lysates; Mouse E13.5, E15.5 and E18.5 heart tissue lysates. IP: HeLa and MEF whole cell lysates.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR20922-47

  • Isotype

    IgG

  • Research areas

Images

  • Immunoprecipitation - Anti-ZNHIT1 antibody [EPR20922-47] (ab238125)
    Immunoprecipitation - Anti-ZNHIT1 antibody [EPR20922-47] (ab238125)

    ZNHIT1 was immunoprecipitated from MEF (mouse embryonic fibroblast) whole cell lysate with ab238125 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab238125 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.

    Lane 1: MEF whole cell lysate 10 ug

    Lane 2: ab238125 IP in MEF whole cell lysate

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab238125 in MEF whole cell lysate

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 3 minutes.

  • Western blot - Anti-ZNHIT1 antibody [EPR20922-47] (ab238125)
    Western blot - Anti-ZNHIT1 antibody [EPR20922-47] (ab238125)
    All lanes : Anti-ZNHIT1 antibody [EPR20922-47] (ab238125) at 1/1000 dilution

    Lane 1 : HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate
    Lane 2 : MEF (mouse embryonic fibroblast (immortalized)) whole cell lysate
    Lane 3 : C2C12 (mouse myoblasts myoblast) whole cell lysate
    Lane 4 : HEK-293T (human embryonic kidney epithelial cell) whole cell lysate
    Lane 5 : PC-12 (rat adrenal gland pheochromocytoma ) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/100000 dilution

    Predicted band size: 18 kDa
    Observed band size: 18 kDa



    Blocking and dilution buffer: 5% NFDM/TBST.

    Exposure time: 70 seconds.

  • Immunoprecipitation - Anti-ZNHIT1 antibody [EPR20922-47] (ab238125)
    Immunoprecipitation - Anti-ZNHIT1 antibody [EPR20922-47] (ab238125)

    ZNHIT1 was immunoprecipitated from 0.35 mg HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate with ab238125 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab238125 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.

    Lane 1: HeLa whole cell lysate 10 ug

    Lane 2: ab238125 IP in HeLa whole cell lysate

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab238125 in HeLa whole cell lysate

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 76 seconds.

     

  • Western blot - Anti-ZNHIT1 antibody [EPR20922-47] (ab238125)
    Western blot - Anti-ZNHIT1 antibody [EPR20922-47] (ab238125)
    All lanes : Anti-ZNHIT1 antibody [EPR20922-47] (ab238125) at 1/1000 dilution

    Lane 1 : Wild-type mouse E13.5 heart tissue lysate
    Lane 2 : ZNHIT1 conditional knockout mouse E13.5 heart tissue lysate
    Lane 3 : Wild-type mouse E15.5 heart tissue lysate
    Lane 4 : ZNHIT1 conditional knockout mouse E15.5 heart tissue lysate
    Lane 5 : Wild-type mouse E18.5 heart tissue lysate
    Lane 6 : ZNHIT1 conditional knockout mouse E18.5 heart tissue lysate

    Lysates/proteins at 20 µl per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/50000 dilution

    Predicted band size: 18 kDa
    Observed band size: 18 kDa



    Blocking and dilution buffer: 5% NFDM/TBST.

    Exposure time: 3 minutes.

    Lysates were kindly provided by an anonymous collaborator.

  • Anti-ZNHIT1 antibody [EPR20922-47] (ab238125)
    Anti-ZNHIT1 antibody [EPR20922-47] (ab238125)

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