Anti-ZNF312/FEZF2 antibody (ab69436)
Key features and details
- Rabbit polyclonal to ZNF312/FEZF2
- Suitable for: ICC/IF, WB
- Reacts with: Mouse, Rat
- Isotype: IgG
Overview
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Product name
Anti-ZNF312/FEZF2 antibody
See all ZNF312/FEZF2 primary antibodies -
Description
Rabbit polyclonal to ZNF312/FEZF2 -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, WBmore details -
Species reactivity
Reacts with: Mouse, Rat
Predicted to work with: Cow, Human
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Immunogen
Synthetic peptide corresponding to Mouse ZNF312/FEZF2 aa 50-150 conjugated to keyhole limpet haemocyanin.
(Peptide available asab101386) -
Positive control
- This antibody gave a positive signal in Mouse spinal cord tissue lysate.
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General notes
This product was previously labelled as ZNF312
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Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab69436 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application Abreviews Notes ICC/IF Use a concentration of 1 µg/ml. WB Use a concentration of 1 µg/ml. Detects a band of approximately 48 kDa (predicted molecular weight: 48 kDa). Target
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Relevance
ZNF312, also known as Fezf2 or Fez-like, is a zinc finger protein that acts as a transcriptional repressor during the development of corticospinal motor neurons and other subcerebral projection neurons. ZNF312 is expressed by early progenitor cells in the ventricular zone. It regulates the fate choice of subcortical projection neurons in the developing cerebral cortex. This protein is expressed in the developing cortical plate during early embryonic development. During late embryonic development and early postnatal development, ZNF312 expression disappears from the cortical progenitors and becomes restricted to the subplate nad the prospective layer V and VI pyramidal neurons. -
Cellular localization
Nuclear -
Database links
- Entrez Gene: 55079 Human
- Entrez Gene: 54713 Mouse
- Entrez Gene: 305719 Rat
- Omim: 607414 Human
- SwissProt: Q8TBJ5 Human
- SwissProt: Q9ESP5 Mouse
- Unigene: 241523 Human
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Alternative names
- Fez antibody
- FEZ family zinc finger protein 2 antibody
- FEZF 2 antibody
see all
Images
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Western blot - Anti-ZNF312/FEZF2 antibody (ab69436)Anti-ZNF312/FEZF2 antibody (ab69436) at 1 µg/ml + Spinal Cord (Mouse) Tissue Lysate at 10 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 48 kDa
Observed band size: 48 kDa
Additional bands at: 17 kDa, 33 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 8 minutes -
Immunocytochemistry/ Immunofluorescence - Anti-ZNF312/FEZF2 antibody (ab69436)ICC/IF image of ab69436 stained PC12 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab69436, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Protocols
Datasheets and documents
References (3)
ab69436 has been referenced in 3 publications.
- Chen Z et al. Loss of Fezf2 promotes malignant progression of bladder cancer by regulating the NF-?B signaling pathway. Lab Invest 98:1225-1236 (2018). PubMed: 29925938
- Qu C et al. Transient Expression of Fez Family Zinc Finger 2 Protein Regulates the Brn3b Gene in Developing Retinal Ganglion Cells. J Biol Chem 291:7661-8 (2016). PubMed: 26861874
- Shu XS et al. FEZF2, a novel 3p14 tumor suppressor gene, represses oncogene EZH2 and MDM2 expression and is frequently methylated in nasopharyngeal carcinoma. Carcinogenesis 34:1984-93 (2013). WB . PubMed: 23677067
Images
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Western blot - Anti-ZNF312/FEZF2 antibody (ab69436)Anti-ZNF312/FEZF2 antibody (ab69436) at 1 µg/ml + Spinal Cord (Mouse) Tissue Lysate at 10 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 48 kDa
Observed band size: 48 kDa
Additional bands at: 17 kDa, 33 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 8 minutes
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Immunocytochemistry/ Immunofluorescence - Anti-ZNF312/FEZF2 antibody (ab69436)ICC/IF image of ab69436 stained PC12 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab69436, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
