Anti-ZNF24 antibody [EPR13975] - BSA and Azide free (ab250134)
![Anti-ZNF24 antibody [EPR13975] - BSA and Azide free (ab250134)](https://www.abcam.com/ps/products/250/ab250134/Images/ab250134-2-anti-znf24-antibody-epr13975-bsa-and-azide-free-flow-cytometry.jpg "Anti-ZNF24 antibody [EPR13975] - BSA and Azide free (ab250134)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR13975] to ZNF24 - BSA and Azide free
- Suitable for: WB, Flow Cyt
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Anti-ZNF24 antibody [EPR13975] - BSA and Azide free
See all ZNF24 primary antibodies -
Description
Rabbit monoclonal [EPR13975] to ZNF24 - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: WB, Flow Cytmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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General notes
Ab250134 is the carrier-free version of ab180121. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab250134 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Affinity purified -
Clonality
Monoclonal -
Clone number
EPR13975 -
Isotype
IgG -
Research areas
Images
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Flow Cytometry - Anti-ZNF24 antibody [EPR13975] - BSA and Azide free (ab250134)This data was developed using ab180121, the same antibody clone in a different buffer formulation.Flow cytometric analysis of HeLa cells (paraformaldehyde-fixed, 2%) labeling ZNF24 with ab180121 at 1/10 dilution (red) or a rabbit IgG (negative) (green), followed by secondary Goat anti rabbit IgG (FITC) at 1/75 dilution. Isotype control Rabbit IgG.
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![Western blot - Anti-ZNF24 antibody [EPR13975] - BSA and Azide free (ab250134)](https://www.abcam.com/ps/products/250/ab250134/Images/ab250134-398235-anti-znf24-antibody-epr13975-bsa-and-azide-free-western-blot.jpg)
Western blot - Anti-ZNF24 antibody [EPR13975] - BSA and Azide free (ab250134)All lanes : Anti-ZNF24 antibody [EPR13975] (ab180121) at 1/10000 dilution
Lane 1 : Jurkat cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : 293 cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 42 kDa
Observed band size: 50 kDa why is the actual band size different from the predicted?This data was developed using ab180121, the same antibody clone in a different buffer formulation.
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![Anti-ZNF24 antibody [EPR13975] - BSA and Azide free (ab250134)](https://www.abcam.com/ps/products/250/ab250134/Images/ab250134-3-benefits-of-recombinant-antibodies.png)
Anti-ZNF24 antibody [EPR13975] - BSA and Azide free (ab250134)
