Antibodies, Proteins, Kits and Reagents for Life Science

298 185 ₸ Product size

100 µl 298 185 ₸

Order now and get it on Tuesday March 02, 2021

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR7790] to Zic2
Suitable for: WB, ICC, Flow Cyt
Reacts with: Mouse, Rat, Human

Product name

Anti-Zic2 antibody [EPR7790]
See all Zic2 primary antibodies
Description

Rabbit monoclonal [EPR7790] to Zic2
Host species

Rabbit
Specificity

This antibody detects a 70kDa extra band in some cell lines.

Tested Applications & Species

Application	Species
Flow Cyt	Human
ICC	Human
WB	Mouse Rat Human

See all applications and species data

Immunogen

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: Human mouse and rat fetal brain tissue lysate, C6' Neuro-2a, PANC-1, SK-OV-3 and U-87 MG; Human fetal brain, Human cerebellum and Mouse embryo tissue lysate ICC: SK-OV-3 cells. Flow cyt: SH-SY5Y cells
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR7790
Isotype

IgG
Research areas

Western blot - Anti-Zic2 antibody [EPR7790] (ab150404)

All lanes : Anti-Zic2 antibody [EPR7790] (ab150404) at 1/2000 dilution

Lane 1 : Human fetal brain tissue lysate
Lane 2 : SH-SY5Y (Human neuroblastoma epithelial cell) whole cell lysate
Lane 3 : C6 (Rat glial tumor glial cell) whole cell lysate
Lane 4 : Neuro-2a (Mouse neuroblastoma neuroblast) whole cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051)

Predicted band size: 55 kDa
Observed band size: 56 kDa
why is the actual band size different from the predicted?

Exposure time: 3 minutes

Diluting/Blocking buffer and concentration: 5% NFDM/TBST

It detects an 70kDa extra band in some cell lines.
Immunocytochemistry - Anti-Zic2 antibody [EPR7790] (ab150404)

Immunocytochemistry analysis of SK-OV-3 (human ovarian cancer epithelial cell)cells labelling Zic2 with Ab150404 at 2.5 μg/ml. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) at 1/1000 was used as the secondary antibody (green). Cells were counterstained with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (ab195889) at 1/200 dilution (red). Nuclear DNA was labelled with DAPI (blue).
Western blot - Anti-Zic2 antibody [EPR7790] (ab150404)

All lanes : Anti-Zic2 antibody [EPR7790] (ab150404) at 1/500 dilution

Lane 1 : Human fetal brain tissue lysate
Lane 2 : PANC-1 (Human pancreatic epithelioid carcinoma epithelial cell) whole cell lysate
Lane 3 : SK-OV-3 (Human ovarian cancer epithelial cell) whole cell lysate
Lane 4 : U-87 MG (Human glioblastoma-astrocytoma epithelial cell) whole cell lysate
Lane 5 : Mouse embryo tissue lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051)

Predicted band size: 55 kDa
Observed band size: 56 kDa why is the actual band size different from the predicted?

Exposure time:

Lane1: 3.25 seconds
Lane2: 20 seconds
Lane3: 5.5 seconds
Lane4-5: 60 seconds

Diluting/Blocking buffer and concentration: 5% NFDM/TBST

ab150404 detects an 70kDa extra band in some cell lines. The expression profile observed in U-87 MG is consistent withthe literature (PMID: 11038359). Negative control: U-87 MG (PMID: 11038359)
Flow Cytometry - Anti-Zic2 antibody [EPR7790] (ab150404)

Flow Cytometry analysis of SH-SY5Y (Human neuroblastoma epithelial cell) cells labeling Zic2 with purified ab150404 at 1:600 dilution (1 µg/ml) (red). Cells were fixed with 80% methanol. A Goat anti rabbit IgG (Alexa Fluor^® 488) secondary antibody was used at 1:2000 dilution. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).
Western blot - Anti-Zic2 antibody [EPR7790] (ab150404)

Anti-Zic2 antibody [EPR7790] (ab150404) at 1/15000 dilution (Purified) + Human fetal brain lysates at 15 µg with 5% NFDM/TBST

Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)

Predicted band size: 55 kDa
Observed band size: 63 kDa why is the actual band size different from the predicted?
Western blot - Anti-Zic2 antibody [EPR7790] (ab150404)

Anti-Zic2 antibody [EPR7790] (ab150404) at 1/20000 dilution (Purified) + Rat brain lysates at 15 µg with 5% NFDM/TBST

Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)

Predicted band size: 55 kDa
Observed band size: 63 kDa why is the actual band size different from the predicted?
Western blot - Anti-Zic2 antibody [EPR7790] (ab150404)

Anti-Zic2 antibody [EPR7790] (ab150404) at 1/5000 dilution (Purified) + Mouse brain lysates at 15 µg with 5% NFDM/TBST

Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)

Predicted band size: 55 kDa
Observed band size: 63 kDa why is the actual band size different from the predicted?
Flow Cytometry - Anti-Zic2 antibody [EPR7790] (ab150404)

Overlay histogram showing SH-SY5Y cells stained with unpurified ab150404 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody ( unpurified ab150404, 1/100 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor^® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1μg/1x10⁶ cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
Western blot - Anti-Zic2 antibody [EPR7790] (ab150404)

All lanes : Anti-Zic2 antibody [EPR7790] (ab150404) at 1/1000 dilution (unpurified)

Lane 1 : Human fetal brain lysate
Lane 2 : Human cerebellum lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat anti-rabbit HRP at 1/2000 dilution

Predicted band size: 55 kDa
Observed band size: ~63 kDa why is the actual band size different from the predicted?
Anti-Zic2 antibody [EPR7790] (ab150404)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com