Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Zfp281/ZNF281 antibody (ab101318)

Key features and details

  • Rabbit polyclonal to Zfp281/ZNF281
  • Suitable for: WB
  • Reacts with: Mouse
  • Isotype: IgG

Overview

  • Product name

    Anti-Zfp281/ZNF281 antibody
    See all Zfp281/ZNF281 primary antibodies

  • Description

    Rabbit polyclonal to Zfp281/ZNF281

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WBmore details

  • Species reactivity

    Reacts with: Mouse
    Predicted to work with: Rat, Rabbit, Horse, Dog, Human, Chimpanzee, Macaque monkey, Gorilla, Chinese hamster

  • Immunogen

    Synthetic peptide corresponding to Human Zfp281/ZNF281 aa 800 to the C-terminus conjugated to keyhole limpet haemocyanin.
    (Peptide available as ab130905)

  • Positive control

    • This antibody gave a positive signal in a wild type mouse embryonic stem cell lysate, which is absent from a Zfp281/ZNF281 null mouse embryonic stem cell lysate.

  • General notes

     This product was previously labelled as Zfp281

     

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Applications

Our Abpromise guarantee covers the use of ab101318 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 120 kDa (predicted molecular weight: 97 kDa).

Target

  • Function

    Involved in transcriptional regulation. Represses the transcription of a number of genes including gastrin and ornithine decarboxylase. Binds to the G-rich box in the enhancer region of these genes.

  • Sequence similarities

    Belongs to the krueppel C2H2-type zinc-finger protein family.
    Contains 4 C2H2-type zinc fingers.

  • Post-translational
    modifications

    Phosphorylated upon DNA damage, probably by ATM or ATR.

  • Cellular localization

    Nucleus.
  • Information by UniProt

  • Database links

    • Alternative names

      • GC box binding zinc finger protein 1 antibody
      • GC-box-binding zinc finger protein 1 antibody
      • GZP1 antibody
      • Transcription factor ZBP-99 antibody
      • ZBP 99 antibody
      • ZBP99 antibody
      • zfp281 antibody
      • Zinc finger DNA binding protein 99 antibody
      • Zinc finger DNA-binding protein 99 antibody
      • Zinc finger protein 281 antibody
      • ZN281_HUMAN antibody
      • ZNF281 antibody
      • ZNP 99 antibody
      • ZNP 99 transcription factor antibody
      • ZNP99 antibody
      see all

    Images

    • Western blot - Anti-Zfp281/ZNF281 antibody (ab101318)
      Western blot - Anti-Zfp281/ZNF281 antibody (ab101318)
      All lanes : Anti-Zfp281/ZNF281 antibody (ab101318) at 1 µg/ml

      Lane 1 : wild type mouse embryonic stem cell lysate
      Lane 2 : Zfp281/ZNF281 null mouse embryonic stem cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

      Developed using the ECL technique.

      Performed under reducing conditions.

      Predicted band size: 97 kDa
      Observed band size: 120 kDa
      why is the actual band size different from the predicted?
      Additional bands at: 78 kDa. We are unsure as to the identity of these extra bands.


      Exposure time: 4 minutes

    References (6)

    Publishing research using ab101318? Please let us know so that we can cite the reference in this datasheet.

    ab101318 has been referenced in 6 publications.

    • Nicolai S  et al. ZNF281/Zfp281 is a target of miR-1 and counteracts muscle differentiation. Mol Oncol 14:294-308 (2020). PubMed: 31782884
    • Guo J  et al. The transcription factor Zfp281 sustains CD4+ T lymphocyte activation through directly repressing Ctla-4 transcription. Cell Mol Immunol N/A:N/A (2019). PubMed: 31511645
    • Ishiuchi T  et al. Zfp281 Shapes the Transcriptome of Trophoblast Stem Cells and Is Essential for Placental Development. Cell Rep 27:1742-1754.e6 (2019). PubMed: 31067460
    • Huang X  et al. Zfp281 is essential for mouse epiblast maturation through transcriptional and epigenetic control of Nodal signaling. Elife 6:N/A (2017). PubMed: 29168693
    • Fidalgo M  et al. Zfp281 Coordinates Opposing Functions of Tet1 and Tet2 in Pluripotent States. Cell Stem Cell 19:355-69 (2016). PubMed: 27345836
    • Fidalgo M  et al. Zfp281 mediates Nanog autorepression through recruitment of the NuRD complex and inhibits somatic cell reprogramming. Proc Natl Acad Sci U S A 109:16202-7 (2012). WB, ChIP, IP . PubMed: 22988117

    Images

    • Western blot - Anti-Zfp281/ZNF281 antibody (ab101318)
      Western blot - Anti-Zfp281/ZNF281 antibody (ab101318)
      All lanes : Anti-Zfp281/ZNF281 antibody (ab101318) at 1 µg/ml

      Lane 1 : wild type mouse embryonic stem cell lysate
      Lane 2 : Zfp281/ZNF281 null mouse embryonic stem cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

      Developed using the ECL technique.

      Performed under reducing conditions.

      Predicted band size: 97 kDa
      Observed band size: 120 kDa
      why is the actual band size different from the predicted?
      Additional bands at: 78 kDa. We are unsure as to the identity of these extra bands.


      Exposure time: 4 minutes

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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