Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-ZCCHC4 antibody [EPR20492] - BSA and Azide free (ab251525)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR20492] to ZCCHC4 - BSA and Azide free
  • Suitable for: Flow Cyt, WB, IP
  • Knockout validated
  • Reacts with: Human

Overview

  • Product name

    Anti-ZCCHC4 antibody [EPR20492] - BSA and Azide free
    See all ZCCHC4 primary antibodies

  • Description

    Rabbit monoclonal [EPR20492] to ZCCHC4 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: Flow Cyt, WB, IPmore details

  • Species reactivity

    Reacts with: Human
    Predicted to work with: Non human primates

  • Immunogen

    This product was produced with the following immunogens:
    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    Ab251525 is the carrier-free version of ab211325. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab251525 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.

  • Storage buffer

    pH: 7.2
    Constituent: PBS

  • Carrier free

    Yes
  • Concentration information loading...

  • Clonality

    Monoclonal

  • Clone number

    EPR20492

  • Isotype

    IgG

  • Research areas

Images

  • Western blot - Anti-ZCCHC4 antibody [EPR20492] - BSA and Azide free (ab251525)
    Western blot - Anti-ZCCHC4 antibody [EPR20492] - BSA and Azide free (ab251525)
    All lanes : Anti-ZCCHC4 antibody [EPR20492] (ab211325) at 1/1000 dilution

    Lane 1 : Wild type HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
    Lane 2 : ZCCHC4 knockout HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Developed using the ECL technique.

    Predicted band size: 59 kDa
    Observed band size: 59 kDa


    Exposure time: 30 seconds


    This data was developed using ab211325, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

    This antibody works best on a semi-dry nitrocellulose membrane transfer system.

    These lysates were kindly provided by Prof Chuan He; University of Chicago.

  • Flow Cytometry - Anti-ZCCHC4 antibody [EPR20492] - BSA and Azide free (ab251525)
    Flow Cytometry - Anti-ZCCHC4 antibody [EPR20492] - BSA and Azide free (ab251525)
    This data was developed using ab211325, the same antibody clone in a different buffer formulation.Flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol permeabilized HeLa (human epithelial cell line from cervix adenocarcinoma) cell line labeling ZCCHC4 with ab211325 at 1/100 dilution (red) compared with a Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) (black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) at 1/2000 dilution was used as the secondary antibody.
  • Flow Cytometry - Anti-ZCCHC4 antibody [EPR20492] - BSA and Azide free (ab251525)
    Flow Cytometry - Anti-ZCCHC4 antibody [EPR20492] - BSA and Azide free (ab251525)
    This data was developed using ab211325, the same antibody clone in a different buffer formulation.Flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol permeabilized HepG2 (human liver hepatocellular carcinoma cell line) cell line labeling ZCCHC4 with ab211325 at 1/100 dilution (red) compared with a Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) (black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) at 1/2000 dilution was used as the secondary antibody.
  • Immunoprecipitation - Anti-ZCCHC4 antibody [EPR20492] - BSA and Azide free (ab251525)
    Immunoprecipitation - Anti-ZCCHC4 antibody [EPR20492] - BSA and Azide free (ab251525)
    This data was developed using ab211325, the same antibody clone in a different buffer formulation.ZCCHC4 was immunoprecipitated from 0.35 mg of HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate with ab211325 at 1/50 dilution. Western blot was performed from the immunoprecipitate using ab211325 at 1/1,000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10,000 dilution. Lane 1: HeLa whole cell lysate 10 µg (Input). Lane 2: ab211325 IP in HeLa whole cell lysate. Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab211325 in HeLa whole cell lysate. Blocking and dilution buffer and concentration: 5% NFDM/TBST. Exposure time: 30 seconds. This antibody works best on a semi-dry nitrocellulose membrane transfer system.
  • Anti-ZCCHC4 antibody [EPR20492] - BSA and Azide free (ab251525)
    Anti-ZCCHC4 antibody [EPR20492] - BSA and Azide free (ab251525)

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