Anti-YTHDF1 antibody [EPR22349-41] - BSA and Azide free (ab253038)
![Anti-YTHDF1 antibody [EPR22349-41] - BSA and Azide free (ab253038)](https://www.abcam.com/ps/products/253/ab253038/Images/ab253038-343609-ab2201624220162IP1.jpg "Anti-YTHDF1 antibody [EPR22349-41] - BSA and Azide free (ab253038)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR22349-41] to YTHDF1 - BSA and Azide free
- Suitable for: WB, IP
- Knockout validated
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Anti-YTHDF1 antibody [EPR22349-41] - BSA and Azide free
See all YTHDF1 primary antibodies -
Description
Rabbit monoclonal [EPR22349-41] to YTHDF1 - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: WB, IPmore details
Unsuitable for: Flow Cyt,ICC/IF or IHC-P -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- IP: HepG2 whole cell lysate.
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General notes
Ab253038 is the carrier-free version of ab220162. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab253038 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR22349-41 -
Isotype
IgG -
Research areas
Images
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Immunoprecipitation - Anti-YTHDF1 antibody [EPR22349-41] - BSA and Azide free (ab253038)
YTHDF1 was immunoprecipitated from 0.35 mg HepG2 (Human hepatocellular carcinoma epithelial cell) whole cell lysate with ab220162 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab220162 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/5000 dilution.
Lane 1: HepG2 whole cell lysate 10 µg (Input).
Lane 2: ab220162 IP in HepG2 whole cell lysate.
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab220162 in HepG2 whole cell lysate.
Blocking/Dilution buffer: 5% NFDM/TBST.
Exposure time: 3 seconds.Lysate were made freshly and used in IP test immediately to minimize protein degradation. Incubation time was 2h.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab220162).
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![Anti-YTHDF1 antibody [EPR22349-41] - BSA and Azide free (ab253038)](https://www.abcam.com/ps/products/253/ab253038/Images/ab253038-2-benefits-of-recombinant-antibodies.png)
Anti-YTHDF1 antibody [EPR22349-41] - BSA and Azide free (ab253038)
