Anti-XRCC3 antibody [XRCC3 10F1/6] (ab20254)
![Anti-XRCC3 antibody [XRCC3 10F1/6] (ab20254)](https://www.abcam.com/ps/products/20/ab20254/Images/ab20254-29545-anti-xrcc3-antibody-xrcc3-10f1-6-immunohistochemistry.jpg "Anti-XRCC3 antibody [XRCC3 10F1/6] (ab20254)")
Key features and details
- Mouse monoclonal [XRCC3 10F1/6] to XRCC3
- Suitable for: IHC-P
- Reacts with: Human
- Isotype: IgG1
Overview
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Product name
Anti-XRCC3 antibody [XRCC3 10F1/6]
See all XRCC3 primary antibodies -
Description
Mouse monoclonal [XRCC3 10F1/6] to XRCC3 -
Host species
Mouse -
Tested applications
Suitable for: IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Full length protein (human) which has been his-tagged, overexpressed in E.coli and purified on a talon affinity column under denaturing conditions, followed by gel purification on a SDS-Page.
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Positive control
- Does not recognise XRCC3 in HeLa total extracts- suggest nuclear extracts.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Constituent: PBS -
Concentration information loading... -
Purity
Protein A/G purified -
Clonality
Monoclonal -
Clone number
XRCC3 10F1/6 -
Myeloma
Sp2/0-Ag14 -
Isotype
IgG1 -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-XRCC3 antibody [XRCC3 10F1/6] (ab20254)Ab20254 staining human normal placenta. Staining is localized to nuclear compartment.
Left panel: with primary antibody at 4 ug/ml. Right panel: isotype control.
Sections were stained using an automated system DAKO Autostainer Plus , at room temperature. Sections were rehydrated and antigen retrieved with the Dako 3-in-1 AR buffer EDTA pH 9.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 minutes and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.
