Anti-Wwp2 antibody (ab103527)
Key features and details
- Rabbit polyclonal to Wwp2
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Wwp2 antibody -
Description
Rabbit polyclonal to Wwp2 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IP HumanWB Human
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Immunogen
Synthetic peptide corresponding to a region between residue 200 and 250 of Human Wwp2 (NP_008945.2).
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Positive control
- HeLa, 293T, Jurkat and mouse NIH3T3 whole cell lysate
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Wwp2 antibody (ab103527)All lanes : Anti-Wwp2 antibody (ab103527) at 0.1 µg/ml
Lane 1 : 293T cell lysate at 50 µg
Lane 2 : 293T cell lysate at 15 µg
Lane 3 : 293T cell lysate at 5 µg
Lane 4 : HeLa cell lysate at 50 µg
Lane 5 : Jurkat cell lysate at 50 µg
Predicted band size: 99 kDa
Exposure time: 30 secondsCells were prepared using NETN lysis buffer.
Detection: Chemiluminescence
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Immunoprecipitation - Anti-Wwp2 antibody (ab103527)ab103527 at 1 used at 6 µg/reaction immunoprecipitating Wwp2 in HEK293T cell lysate.
Lane 1: ab103527 + HEK293T cell lysate
Lane 2: Rabbit anti-WWP2 Antibody instead of ab103527 in HEK293T cell lysate
Lane 3: Control IgG instead of ab32562 in HEK293T cell lysate.
For blotting ab103527 was used at 1 µg/mL.
Detection: Chemiluminescence
Exposure time: 30 seconds