Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-WIF1 antibody [EPR9385] - BSA and Azide free (ab249191)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR9385] to WIF1 - BSA and Azide free
  • Suitable for: WB, IP
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-WIF1 antibody [EPR9385] - BSA and Azide free
    See all WIF1 primary antibodies

  • Description

    Rabbit monoclonal [EPR9385] to WIF1 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IPmore details
    Unsuitable for: ICC/IF

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
    (Peptide available as ab171766)

  • General notes

    Ab249191 is the carrier-free version of ab155101. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab249191 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.

  • Storage buffer

    pH: 7.2
    Constituent: PBS

  • Carrier free

    Yes
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR9385

  • Isotype

    IgG

  • Research areas

Images

  • Western blot - Anti-WIF1 antibody [EPR9385] - BSA and Azide free (ab249191)
    Western blot - Anti-WIF1 antibody [EPR9385] - BSA and Azide free (ab249191)
    Anti-WIF1 antibody [EPR9385] (ab155101) at 1/2000 dilution (purified) + Human fetal heart tissue lysate at 20 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 41 kDa
    Observed band size: 41 kDa



    This data was developed using ab155101, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-WIF1 antibody [EPR9385] - BSA and Azide free (ab249191)
    Western blot - Anti-WIF1 antibody [EPR9385] - BSA and Azide free (ab249191)
    Anti-WIF1 antibody [EPR9385] (ab155101) at 1/5000 dilution (purified) + Human fetal lung tissue lysate at 10 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 41 kDa
    Observed band size: 41 kDa



    This data was developed using ab155101, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-WIF1 antibody [EPR9385] - BSA and Azide free (ab249191)
    Western blot - Anti-WIF1 antibody [EPR9385] - BSA and Azide free (ab249191)
    All lanes : Anti-WIF1 antibody [EPR9385] (ab155101) at 1/1000 dilution (purified)

    Lane 1 : Mouse heart tissue lysate
    Lane 2 : Rat heart tissue lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 41 kDa
    Observed band size: 41 kDa



    This data was developed using ab155101, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-WIF1 antibody [EPR9385] - BSA and Azide free (ab249191)
    Western blot - Anti-WIF1 antibody [EPR9385] - BSA and Azide free (ab249191)
    All lanes : Anti-WIF1 antibody [EPR9385] (ab155101) at 1/1000 dilution (unpurified)

    Lane 1 : HT29 cell lysate
    Lane 2 : HeLa cell lysate
    Lane 3 : Human fetal lung tissue lysate

    Lysates/proteins at 10 µg per lane.

    Predicted band size: 41 kDa



    This data was developed using ab155101, the same antibody clone in a different buffer formulation.

  • Immunoprecipitation - Anti-WIF1 antibody [EPR9385] - BSA and Azide free (ab249191)
    Immunoprecipitation - Anti-WIF1 antibody [EPR9385] - BSA and Azide free (ab249191)

    This data was developed using ab155101, the same antibody clone in a different buffer formulation.

    Purified ab155101 (1/60) immunoprecipitating WIF1 in human fetal lung whole cell lysate (Lane +). For western blotting an Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG (1/500) was used as the secondary antibody.

    Lane (-): Rabbit monoclonal IgG (ab172730) instead of ab155101 in Human fetal lung whole cell lysate.

    Blocking and dilution buffer: 5% NFDM/TBST.

  • Anti-WIF1 antibody [EPR9385] - BSA and Azide free (ab249191)
    Anti-WIF1 antibody [EPR9385] - BSA and Azide free (ab249191)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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