Anti-WDR79 antibody (ab224444)
Key features and details
- Rabbit polyclonal to WDR79
- Suitable for: IHC-P, ICC/IF, WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-WDR79 antibody
See all WDR79 primary antibodies -
Description
Rabbit polyclonal to WDR79 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, ICC/IF, WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment corresponding to Human WDR79 aa 100-250.
Database link: Q9BUR4 -
Positive control
- WB: WDR79 over-expression - HEK-293T whole cell lysate; IHC-P: Human Fallopian tube, kidney and skin tissue; ICC: A431 cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-WDR79 antibody (ab224444)All lanes : Anti-WDR79 antibody (ab224444) at 0.4 µg/ml
Lane 1 : Control (vector only transfected) - HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 2 : WDR79 over-expression - HEK-293T whole cell lysate, Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa)
Predicted band size: 59 kDa
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-WDR79 antibody (ab224444)Immunohistochemical analysis of human Fallopian tube tissue labeling WDR79 in the nucleus of glandular cells with ab224444 at 1/20 dilution.
Performed heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunocytochemistry/ Immunofluorescence - Anti-WDR79 antibody (ab224444)Immunofluorescent analysis of A431 (human epidermoid carcinoma cell line) cells labeling WDR79 (green) in nuclear bodies with ab224444 at 2 µg/ml.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-WDR79 antibody (ab224444)Immunohistochemical analysis of human kidney tissue labeling WDR79 in the nucleus of cells in tubules with ab224444 at 1/20 dilution.
Performed heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-WDR79 antibody (ab224444)Immunohistochemical analysis of human skin tissue labeling WDR79 in the nucleus of squamous epithelial cells with ab224444 at 1/20 dilution.
Performed heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-WDR79 antibody (ab224444)Immunohistochemical analysis of human liver tissue labeling WDR79 with ab224444 at 1/20 dilution. No positivity as expected.
Performed heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
