Anti-WDR37 antibody (ab235046)
Key features and details
- Rabbit polyclonal to WDR37
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-WDR37 antibody -
Description
Rabbit polyclonal to WDR37 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Xenopus laevis, Orangutan, Xenopus tropicalis
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Immunogen
Recombinant full length protein corresponding to Human WDR37 aa 1 to the C-terminus.
Database link: Q8CBE3 -
Positive control
- WB: HepG2 cell lysate. IHC-P: Human colon cancer and appendix tissue.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.03% Proclin 300 -
Concentration information loading... -
Purity
Protein G purified -
Purification notes
Purity >95%. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-WDR37 antibody (ab235046)Anti-WDR37 antibody (ab235046) at 1/500 dilution + HepG2 (human liver hepatocellular carcinoma cell line) cell lysate
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-WDR37 antibody (ab235046)Paraffin-embedded human colon cancer tissue stained for WDR37 using ab235046 at 1/500 dilution in immunohistochemical analysis.
After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum for 30 minutes at room temperature. Primary antibody (1% BSA) was incubated at 4°C overnight. The primary antibody was detected by a biotinylated secondary antibody and visualized using a HRP conjugated SP system.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-WDR37 antibody (ab235046)Paraffin-embedded human appendix tissue stained for WDR37 using ab235046 at 1/500 dilution in immunohistochemical analysis.
After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum for 30 minutes at room temperature. Primary antibody (1% BSA) was incubated at 4°C overnight. The primary antibody was detected by a biotinylated secondary antibody and visualized using a HRP conjugated SP system.