Anti-VTA1 antibody (ab279583)
Key features and details
- Rabbit polyclonal to VTA1
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-VTA1 antibody
See all VTA1 primary antibodies -
Description
Rabbit polyclonal to VTA1 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt HumanWB Human
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Immunogen
Synthetic peptide corresponding to Human VTA1. corresponding to the center region
Database link: Q9NP79 -
Positive control
- WB: H1299, HeLa, Molt4 and HepG2 Whole Cell Lysates. IP: H1299 Whole Cell Lysate.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.03% Proclin 300
Constituent: 99.97% PBS -
Concentration information loading... -
Purification notes
Protein A & Antigen Affinity chromatography -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-VTA1 antibody (ab279583)All lanes : Anti-VTA1 antibody (ab279583) at 1/500 dilution
Lane 1 : H1299 Whole Cell Lysate
Lane 2 : HeLa Whole Cell Lysate
Lane 3 : Molt4 Whole Cell Lysate
Lane 4 : HepG2 Whole Cell Lysate
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution
Predicted band size: 34 kDa
Observed band size: 38 kDa why is the actual band size different from the predicted?Developed using the ECL technique.
Performed under reducing conditions.
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Immunoprecipitation - Anti-VTA1 antibody (ab279583)VTA1 was immunoprecipitated using:
Lane 1: 0.5 mg H1299 Whole Cell Lysate
4 µL anti-VTA1 rabbit polyclonal antibody (ab279583) and 60 μg of Immunomagnetic beads Protein A/G.
Primary antibody:
ab279583 Anti-VTA1 rabbit polyclonal antibody,at 1:100 dilution
Secondary antibody:
Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.