Antibodies, Proteins, Kits and Reagents for Life Science

288 134 ₸ Product size

100 µl 288 134 ₸ 1 ml 465 705 ₸

Order now and get it on Tuesday March 02, 2021

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [SP235] to VPAC2
Suitable for: Flow Cyt, IHC-P
Reacts with: Mouse, Rat, Human

Product name

Anti-VPAC2 antibody [SP235]
See all VPAC2 primary antibodies
Description

Rabbit monoclonal [SP235] to VPAC2
Host species

Rabbit

Tested Applications & Species

Application	Species
Flow Cyt	Human
IHC-P	Mouse Rat Human

See all applications and species data

Immunogen

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
Positive control
- IHC-P: Human liver, hepatocellular carcinoma, liver cirrhosis, Human tonsil, Mouse spleen, and Rat spleen tissue.
General notes
This product is FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.

This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.60
Preservative: 0.1% Sodium azide
Constituents: PBS, 1% BSA
Concentration information loading...
Purity

Immunogen affinity purified
Purification notes

Purified from TCS by protein A/G.
Clonality

Monoclonal
Clone number

SP235
Isotype

IgG
Research areas

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VPAC2 antibody [SP235] (ab183334)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Rat spleen tissue sections labeling VPAC2 with ab183334 at 1/100 dilution (0.96 µg/ml). Heat mediated antigen retrieval was performed Heat mediated antigen retrieval with sodium citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VPAC2 antibody [SP235] (ab183334)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Mouse spleen tissue sections labeling VPAC2 with ab183334 at 1/100 dilution (0.96 µg/ml). Heat mediated antigen retrieval was performed Heat mediated antigen retrieval with sodium citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VPAC2 antibody [SP235] (ab183334)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human tonsil tissue sections labeling VPAC2 with ab183334 at 1/100 dilution (0.96 µg/ml). Heat mediated antigen retrieval was performed Heat mediated antigen retrieval with sodium citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
Flow Cytometry - Anti-VPAC2 antibody [SP235] (ab183334)

Flow Cytometry analysis of Jurkat (Human T cell leukemia T lymphocyte) cells labeling VPAC2 with purified ab183334 at 1/20 dilution (5 µg/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077) secondary antibody was used at 1/2000 dilution. Isotype control - Rabbit monoclonal IgG (ab172730) / Black. Unlabeled control - Unlabelled cells / blue.
Flow Cytometry - Anti-VPAC2 antibody [SP235] (ab183334)

Flow cytometric analysis of Jurkat (Human T cell leukemia cell line from peripheral blood) cells labeling VPAC2 using ab183334 (green) compare to negative control of rabbit IgG (blue).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VPAC2 antibody [SP235] (ab183334)

Immunohistochemical analysis of formalin-fixed, paraffin-embedded human tonsil tissue labeling VPAC2 using ab183334 at a 1/100 dilution.
Anti-VPAC2 antibody [SP235] (ab183334)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com