Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-VKORC1 antibody [EPR20245] (ab206656)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR20245] to VKORC1
  • Suitable for: WB, Flow Cyt, IP, IHC-P, ICC/IF
  • Reacts with: Mouse, Human

Overview

  • Product name

    Anti-VKORC1 antibody [EPR20245]
    See all VKORC1 primary antibodies

  • Description

    Rabbit monoclonal [EPR20245] to VKORC1

  • Host species

    Rabbit

  • Specificity

    This antibody is unsuitable for IHC in human.

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Human
    IHC-P
    Mouse
    IP
    Human
    WB
    Mouse
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Human fetal kidney and fetal liver lysates; Mouse liver, lung and pancreas lysates; HepG2 and A549 whole cell lysates. IHC-P: Mouse liver tissue. ICC/IF: HepG2 and A549 cells. Flow Cyt: HepG2 cells. IP: HepG2 whole cell lysate.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR20245

  • Isotype

    IgG

  • Research areas

Images

  • Western blot - Anti-VKORC1 antibody [EPR20245] (ab206656)
    Western blot - Anti-VKORC1 antibody [EPR20245] (ab206656)
    All lanes : Anti-VKORC1 antibody [EPR20245] (ab206656) at 1/1000 dilution

    Lane 1 : Human fetal kidney lysate
    Lane 2 : Human fetal liver lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/10000 dilution

    Predicted band size: 18 kDa
    Observed band size: 15 kDa
    why is the actual band size different from the predicted?


    Exposure time: 3 minutes


    Blocking/Dilution buffer: 5% NFDM/TBST.

    The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID: 25753038).

  • Western blot - Anti-VKORC1 antibody [EPR20245] (ab206656)
    Western blot - Anti-VKORC1 antibody [EPR20245] (ab206656)
    All lanes : Anti-VKORC1 antibody [EPR20245] (ab206656) at 1/1000 dilution

    Lane 1 : HepG2 (Human liver hepatocellular carcinoma cell line) whole cell lysate
    Lane 2 : A549 (Human lung carcinoma cell line) whole cell lysate
    Lane 3 : Mouse liver lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 18 kDa
    Observed band size: 15 kDa why is the actual band size different from the predicted?


    Exposure time: 8 seconds


    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-VKORC1 antibody [EPR20245] (ab206656)
    Western blot - Anti-VKORC1 antibody [EPR20245] (ab206656)
    All lanes : Anti-VKORC1 antibody [EPR20245] (ab206656) at 1/1000 dilution

    Lane 1 : Mouse lung lysate
    Lane 2 : Mouse pancreas lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 18 kDa
    Observed band size: 15 kDa why is the actual band size different from the predicted?


    Exposure time: 3 minutes


    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VKORC1 antibody [EPR20245] (ab206656)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VKORC1 antibody [EPR20245] (ab206656)

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue labeling VKORC1 with ab206656 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasmic staining on hepatocytes of mouse liver is observed [PMID: 25747820] [PMID:14765194]. Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunocytochemistry/ Immunofluorescence - Anti-VKORC1 antibody [EPR20245] (ab206656)
    Immunocytochemistry/ Immunofluorescence - Anti-VKORC1 antibody [EPR20245] (ab206656)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized A549 (Human lung carcinoma cell line) cells labeling VKORC1 with ab206656 at 1/100 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic staining on A549 cell line.

    The nuclear counter stain is DAPI (blue). Tubulin is detected with ab195889 (Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594)) at 1/200 dilution (red).

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) at 1/1000 dilution.

  • Immunocytochemistry/ Immunofluorescence - Anti-VKORC1 antibody [EPR20245] (ab206656)
    Immunocytochemistry/ Immunofluorescence - Anti-VKORC1 antibody [EPR20245] (ab206656)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HepG2 (Human liver hepatocellular carcinoma cell line) cells labeling VKORC1 with ab206656 at 1/100 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic staining on HepG2 cell line.

    The nuclear counter stain is DAPI (blue). Tubulin is detected with ab195889 (Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594)) at 1/200 dilution (red).

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) at 1/1000 dilution.

  • Flow Cytometry - Anti-VKORC1 antibody [EPR20245] (ab206656)
    Flow Cytometry - Anti-VKORC1 antibody [EPR20245] (ab206656)

    Flow cytometric analysis of 4% paraformaldehyde-fixed HepG2 (Human liver hepatocellular carcinoma cell line) cells labeling VKORC1 with ab206656 at 1/60 dilution (red) compared with a rabbit monoclonal IgG isotype control (ab172730; black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody; blue). Goat anti rabbit IgG (Alexa Fluor® 488) at 1/2000 dilution was used as the secondary antibody.

  • Immunoprecipitation - Anti-VKORC1 antibody [EPR20245] (ab206656)
    Immunoprecipitation - Anti-VKORC1 antibody [EPR20245] (ab206656)

    VKORC1 was immunoprecipitated from 0.35 mg of HepG2 (Human liver hepatocellular carcinoma cell line) whole cell lysate with ab206656 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab206656 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.

    Lane 1: HepG2 whole cell lysate, 10 μg (Input).

    Lane 2: ab206656 IP in HepG2 whole cell lysate.

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab206656 in HepG2 whole cell lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 30 seconds.

  • Anti-VKORC1 antibody [EPR20245] (ab206656)
    Anti-VKORC1 antibody [EPR20245] (ab206656)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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