Anti-VGLUT2 antibody [8G9.2] (ab79157)
![Anti-VGLUT2 antibody [8G9.2] (ab79157)](https://www.abcam.com/ps/products/79/ab79157/Images/ab79157-94655-anti-vglut2-antibody-8g92-immunohistochemistry.jpg "Anti-VGLUT2 antibody [8G9.2] (ab79157)")
Key features and details
- Mouse monoclonal [8G9.2] to VGLUT2
- Suitable for: ICC/IF, IHC-FrFl, WB
- Reacts with: Mouse, Rat
- Isotype: IgG1
Overview
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Product name
Anti-VGLUT2 antibody [8G9.2]
See all VGLUT2 primary antibodies -
Description
Mouse monoclonal [8G9.2] to VGLUT2 -
Host species
Mouse -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF RatIHC-FrFl MouseWB Rat
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Immunogen
Recombinant full length protein corresponding to Rat VGLUT2.
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Positive control
- Floating mouse brain tissue sections.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.60
Preservative: 0.1% Sodium azide
Constituents: 1.45% Sodium chloride, 0.328% Sodium phosphate -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
8G9.2 -
Isotype
IgG1 -
Research areas
Images
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Immunohistochemistry - Free Floating - Anti-VGLUT2 antibody [8G9.2] (ab79157)ab79157, at 1/300 dilution, staining VGLUT2 by Immunohistochemistry using ABC with NiDAB on floating mouse brain tissue sections.
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![Immunocytochemistry/ Immunofluorescence - Anti-VGLUT2 antibody [8G9.2] (ab79157)](https://www.abcam.com/ps/products/79/ab79157/Images/ab79157-94654-anti-vglut2-antibody-8g92-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-VGLUT2 antibody [8G9.2] (ab79157) This image is courtesy of Ruma Raha-Chowdhury, University Of Cambridge, United KingdomImmunocytochemistical detection of VGLUT2 using antibody ab79157 on rat primary hippocampal neuronal culture. Fixative: Paraformaldehyde. Permeabilization: Triton 0.3% in 0.1X PBS. Blocking step: 10% Serum as blocking agent for 20 mins @ 24°C. Primary Antibody: 1 µg/ml for 1 hour @ 24°C in Donkey serum in 0.1%PBS plus Triton 0.3%. Secondary Antibody: Alexa Fluor® 568 Conjugation (1/1000). The submitted image shows ab79157 staining rat primary hippocampal neurons.
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![Western blot - Anti-VGLUT2 antibody [8G9.2] (ab79157)](https://www.abcam.com/ps/products/79/ab79157/Images/ab79157-94653-anti-vglut2-antibody-8g92-western-blot.jpg)
Western blot - Anti-VGLUT2 antibody [8G9.2] (ab79157)All lanes : Anti-VGLUT2 antibody [8G9.2] (ab79157) at 1 µg/ml
Lane 1 : Rat Cortex Tissue Lysate
Lane 2 : Hippocampus (Mouse) Tissue Lysate
Lane 3 : Rat Substantia Nigra Tissue Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 64 kDa
Observed band size: 63 kDa why is the actual band size different from the predicted?
Additional bands at: 50 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 12 minutes
