Anti-VGluT1 antibody (ab272913)
Key features and details
- Rabbit polyclonal to VGluT1
- Suitable for: IHC-Fr, WB, ICC/IF, IHC-P
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-VGluT1 antibody
See all VGluT1 primary antibodies -
Description
Rabbit polyclonal to VGluT1 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-Fr, WB, ICC/IF, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Recombinant fragment within Mouse VGluT1 (C terminal). The exact sequence is proprietary.
Database link: Q3TXX4 -
Positive control
- WB: Mouse and rat brain tissue extracts. ICC/IF: DIV14 rat E18 primary cortical neurons cells. IHC-P: Mouse cerebellum tissue, rat brain tissue. IHC-Fr: Adult mouse cerebellum tissue.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 78% PBS, 1% BSA, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VGluT1 antibody (ab272913)
Paraffin-embedded mouse cerebellum tissue stained for VGluT1 using ab272913 at 1/500 dilution in immunohistochemical analysis. Antigen retrieval using citrate buffer, pH 6, 15 minutes.
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Western blot - Anti-VGluT1 antibody (ab272913)All lanes : Anti-VGluT1 antibody (ab272913) at 1/1000 dilution
Lane 1 : Mouse brain tissue extracts
Lane 2 : Rat brain tissue extracts
Lysates/proteins at 50 µg per lane.7.5% SDS-PAGE
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Immunocytochemistry/ Immunofluorescence - Anti-VGluT1 antibody (ab272913)4% PFA-fixed DIV14 rat E18 primary cortical neurons cells stained for VGluT1 using ab272913 at 1/500 dilution in ICC/IF.
Red: Beta tubulin.
Blue: DAPI.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VGluT1 antibody (ab272913)Paraffin-embedded rat brain tissue stained for VGluT1 using ab272913 at 1/500 dilution in immunohistochemical analysis. Antigen retrieval using citrate buffer, pH 6, 15 minutes.
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Immunohistochemistry (Frozen sections) - Anti-VGluT1 antibody (ab272913)Frozen sections of adult mouse cerebellum tissue stained for VGluT1 using ab272913 at 1/250 dilution in immunohistochemical analysis.
Red: Beta tubulin.
Blue: DAPI.