Anti-Vav proteins antibody [EP482Y] - BSA and Azide free (ab247298)
![Anti-Vav proteins antibody [EP482Y] - BSA and Azide free (ab247298)](https://www.abcam.com/ps/products/247/ab247298/Images/ab247298-402242-anti-vav-proteins-antibody-ep482y-bsa-and-azide-free-western-blot.jpg "Anti-Vav proteins antibody [EP482Y] - BSA and Azide free (ab247298)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP482Y] to Vav proteins - BSA and Azide free
- Suitable for: ICC/IF, Flow Cyt, WB
- Reacts with: Human
Overview
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Product name
Anti-Vav proteins antibody [EP482Y] - BSA and Azide free
See all Vav proteins primary antibodies -
Description
Rabbit monoclonal [EP482Y] to Vav proteins - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, Flow Cyt, WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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General notes
ab247298 is the carrier-free version of ab40875 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
Ab247298 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EP482Y -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Vav proteins antibody [EP482Y] - BSA and Azide free (ab247298)
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![Flow Cytometry - Anti-Vav proteins antibody [EP482Y] - BSA and Azide free (ab247298)](https://www.abcam.com/ps/products/247/ab247298/Images/ab247298-1-anti-vav-proteins-antibody-ep482y-bsa-and-azide-free-flow-cytometry.jpg)
Flow Cytometry - Anti-Vav proteins antibody [EP482Y] - BSA and Azide free (ab247298)This data was developed using ab40875, the same antibody clone in a different buffer formulation.Overlay histogram showing Jurkat cells stained with ab40875 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab40875, 1/100 dilution) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1µg/1x106 cells) used under the same conditions. Unlabelled sample (blue line). Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
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![Anti-Vav proteins antibody [EP482Y] - BSA and Azide free (ab247298)](https://www.abcam.com/ps/products/247/ab247298/Images/ab247298-3-benefits-of-recombinant-antibodies.png)
Anti-Vav proteins antibody [EP482Y] - BSA and Azide free (ab247298)
