Anti-vascular Amyloid 1-42 antibody [mOC31] - Conformation-Specific (ab201059)
![Anti-vascular Amyloid 1-42 antibody [mOC31] - Conformation-Specific (ab201059)](https://www.abcam.com/ps/products/201/ab201059/Images/ab201059-315650-anti-vascular-amyloid-1-42-antibody-moc31-conformation-specific-immunohistochemistry.jpg "Anti-vascular Amyloid 1-42 antibody [mOC31] - Conformation-Specific (ab201059)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [mOC31] to vascular Amyloid 1-42 - Conformation-Specific
- Suitable for: Dot blot, IHC-P, IHC-FrFl
- Reacts with: Mouse, Human
Overview
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Product name
Anti-vascular Amyloid 1-42 antibody [mOC31] - Conformation-Specific
See all vascular Amyloid 1-42 primary antibodies -
Description
Rabbit monoclonal [mOC31] to vascular Amyloid 1-42 - Conformation-Specific -
Host species
Rabbit -
Tested applications
Suitable for: Dot blot, IHC-P, IHC-FrFlmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Other Immunogen Type corresponding to Human vascular Amyloid 1-42. Amyloid beta 1-42 fibrils were used as the immunogen.
Database link: P05067 -
Positive control
- beta Amyloid (Aß) 1-42 IHC-P: FFPE human brain Alzheimer
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General notes
This antibody was developed as part of a collaboration between Abcam and Professor Charles Glabe, UC Irvine.
ab201059 recognizes a conformation-specific discontinuous epitope of beta Amyloid that maps to segments EFGRHSGY and ED (Hatami et al. 2014). It specifically recognizes a subset of vascular amyloid that does not colocalize with thioflavin S (Hatami et al. 2014, McLean et al. 2013).
For further information on the immunogen, please refer to Hatami et al. 2014 and Kayed et al. 2007.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
mOC31 -
Isotype
IgG -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-vascular Amyloid 1-42 antibody [mOC31] - Conformation-Specific (ab201059)
IHC image of beta Amyloid staining in a formalin fixed paraffin embedded human Alzheimer brain tissue section, performed on a Leica Bond™ system using the standard protocol F. No antigen retrieval was performed prior to staining. The section was incubated with ab201090 at 1/500 dilution for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
Image showing staining of vascular Amyloid as described in Hatami et al. 2014
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![Dot Blot - Anti-vascular Amyloid 1-42 antibody [mOC31] - Conformation-Specific (ab201059)](https://www.abcam.com/ps/products/201/ab201059/Images/ab201059-251979-anti-vascular-amyloid-1-42-antibody-moc31-conformation-specific-dot-blot.jpg)
Dot Blot - Anti-vascular Amyloid 1-42 antibody [mOC31] - Conformation-Specific (ab201059)Dot blot analysis of beta Amyloid 1-42 labeled with ab201059 at 1/100 dilution.
Lane 1: beta Amyloid (Aβ) 1-40.
Lane 2: beta Amyloid (Aβ) 1-42.
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/5000 dilution was used as secondary antibody.
Blocking and diluting buffer: 5% NFDM/TBST.
Exposure time: 1 minute.Antibody reactivity was assessed using a dot blot, which is a non-quantitative method that maintains the native conformation of beta Amyloid. Beta Amyloid 1-40 and 1-42 peptides underwent the following aggregation conditions before being spotted onto a nitrocellulose membrane and detected using ab201059:
Monomers: 0.3 mg of beta Amyloid peptide was dissolved in 30 µl 100 mM NaOH and incubated at room temperature for 10 minutes. It was then diluted with 970 µl of 1% SDS and boiled for five minutes.
Oligomers: 0.3 mg of beta Amyloid peptide was dissolved in 30 µl 100 mM NaOH and incubated at room temperature for 10 minutes. It was then diluted with 970 µl of 10 mM phosphate buffer pH 7.4 containing 0.02% sodium azide and incubated at room temperature for four days.
Fibrils: 0.3 mg of beta Amyloid peptide was dissolved in 1 ml 50% hexafluoroisopropanol (HFIP) with 0.02% sodium azide. It was then stirred constantly for nine days; the first seven with a cap on and the final two with the cap removed to allow evaporation of the HFIP. Fibrils were then sedimented at 20,000 rpm in a microcentrifuge for 20 minutes and resuspended in 1 ml of PBS + 0.02% sodium azide. -
![Immunohistochemistry - Free Floating - Anti-vascular Amyloid 1-42 antibody [mOC31] - Conformation-Specific (ab201059)](https://www.abcam.com/ps/products/201/ab201059/Images/ab201059-255941-anti-vascular-amyloid-1-42-antibody-moc31-conformation-specific-immunohistochemistry.jpg)
Immunohistochemistry - Free Floating - Anti-vascular Amyloid 1-42 antibody [mOC31] - Conformation-Specific (ab201059) Image courtesy of Professor Charles Glabe, UC IrvineImmunohistochemical staining of human brain tissue from a patient with a diagnosis of Alzheimers disease, male, 81 years, 5 hour post mortem index, tangle stage 5, plaque stage B, mini mental status exam score 12. Sections were cut using a vibratome. No antigen retrieval was performed. Free floating sections were stained using using ab201059 at a dilution of 50 ng/mL. The secondary antibody used was a biotinylated goat anti-rabbit at a dilution of 1/225, which was blocked with normal goat serum. The sample was visualized using ABC solution (1 hour incubation) followed by 1-4 minutes of DAB. The sample was mounted and allowed to dry overnight, followed by dehydration in increasingly concentrated ethanol solutions.
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![Dot Blot - Anti-vascular Amyloid 1-42 antibody [mOC31] - Conformation-Specific (ab201059)](https://www.abcam.com/ps/products/201/ab201059/Images/ab201059-251980-anti-vascular-amyloid-1-42-antibody-moc31-conformation-specific-dot-blot.jpg)
Dot Blot - Anti-vascular Amyloid 1-42 antibody [mOC31] - Conformation-Specific (ab201059)Negative control (secondary ab only):
Lane 1: beta Amyloid (Aβ) 1-40.
Lane 2: beta Amyloid (Aβ) 1-42.Primary antibody omitted.
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/5000 dilution was used as secondary antibody.
Blocking and diluting buffer: 5% NFDM/TBST.
Exposure time: 30 seconds. -
![Anti-vascular Amyloid 1-42 antibody [mOC31] - Conformation-Specific (ab201059)](https://www.abcam.com/ps/products/201/ab201059/Images/ab201059-7-benefits-of-recombinant-antibodies.png)
Anti-vascular Amyloid 1-42 antibody [mOC31] - Conformation-Specific (ab201059)