Antibodies, Proteins, Kits and Reagents for Life Science

Anti-VANGL1 antibody [CL0241] (ab242311)

Key features and details

Mouse monoclonal [CL0241] to VANGL1
Suitable for: WB, ICC/IF
Reacts with: Human
Isotype: IgG1

Product name

Anti-VANGL1 antibody [CL0241]
See all VANGL1 primary antibodies
Description

Mouse monoclonal [CL0241] to VANGL1
Host species

Mouse
Tested applications

Suitable for: WB, ICC/IFmore details
Species reactivity

Reacts with: Human
Predicted to work with: Mouse
Immunogen

Recombinant fragment corresponding to Human VANGL1 aa 1-150.
Database link: Q8TAA9

Run BLAST with Run BLAST with
Positive control
- ICC/IF: MCF7 and A431 cells. WB: Control siRNA transfected MCF7 cell lysate. MCF7 cell lysate. VANGL1 over-expression lysate with myc tag in HEK-293T cells lysate.
General notes

The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), PBS
Concentration information loading...
Purity

Protein A purified
Purification notes

Purified from TCS.
Clonality

Monoclonal
Clone number

CL0241
Isotype

IgG1
Research areas

Immunocytochemistry/ Immunofluorescence - Anti-VANGL1 antibody [CL0241] (ab242311)

Immunocytochemistry/Immunofluorescence analysis of MCF7 (Human breast adenocarcinoma cell line) cells labeling VANGL1 using ab242311 at 4 µg/ml (green). Nuclear probe is visualized in blue.
Immunocytochemistry/ Immunofluorescence - Anti-VANGL1 antibody [CL0241] (ab242311)

Immunocytochemistry/Immunofluorescence analysis of A431 (Human epidermoid carcinoma cell line) cells labeling VANGL1 using ab242311 at 4 µg/ml (green). Nuclear probe is visualized in blue, microtubules in red.
Western blot - Anti-VANGL1 antibody [CL0241] (ab242311)

Anti-VANGL1 antibody [CL0241] (ab242311) at 1 µg/ml + MCF7 (Human breast adenocarcinoma cell line) cell lysate
Immunocytochemistry/ Immunofluorescence - Anti-VANGL1 antibody [CL0241] (ab242311)

Immunocytochemistry/Immunofluorescence analysis of A431 (Human epidermoid carcinoma cell line) cells labeling VANGL1 using ab242311 at 4 µg/ml (green). Nuclear probe is visualized in blue.
Western blot - Anti-VANGL1 antibody [CL0241] (ab242311)

All lanes : Anti-VANGL1 antibody [CL0241] (ab242311) at 1 µg/ml

Lane 1 : Vector only transfected HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate
Lane 2 : VANGL1 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag, ~3.1 kDa) in HEK-293T cell lysate
Western blot - Anti-VANGL1 antibody [CL0241] (ab242311)

All lanes : Anti-VANGL1 antibody [CL0241] (ab242311) at 1 µg/ml

Lane 1 : siRNA control transfected MCF7 (Human breast adenocarcinoma cell line) lysate
Lane 2 : siRNA#1 transfected MCF7 lysate
Lane 3 : siRNA#2 transfected MCF7 lysate

Loading control: anti-PPIB.

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