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Anti-VANGL1 antibody [CL0241] (ab242311)

Key features and details

  • Mouse monoclonal [CL0241] to VANGL1
  • Suitable for: WB, ICC/IF
  • Reacts with: Human
  • Isotype: IgG1

Overview

  • Product name

    Anti-VANGL1 antibody [CL0241]
    See all VANGL1 primary antibodies

  • Description

    Mouse monoclonal [CL0241] to VANGL1

  • Host species

    Mouse

  • Tested applications

    Suitable for: WB, ICC/IFmore details

  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse

  • Immunogen

    Recombinant fragment corresponding to Human VANGL1 aa 1-150.
    Database link: Q8TAA9

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • Positive control

    • ICC/IF: MCF7 and A431 cells. WB: Control siRNA transfected MCF7 cell lysate. MCF7 cell lysate. VANGL1 over-expression lysate with myc tag in HEK-293T cells lysate.

  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.20
    Preservative: 0.02% Sodium azide
    Constituents: 40% Glycerol (glycerin, glycerine), PBS
  • Concentration information loading...

  • Purity

    Protein A purified

  • Purification notes

    Purified from TCS.

  • Clonality

    Monoclonal

  • Clone number

    CL0241

  • Isotype

    IgG1

  • Research areas

Images

  • Immunocytochemistry/ Immunofluorescence - Anti-VANGL1 antibody [CL0241] (ab242311)
    Immunocytochemistry/ Immunofluorescence - Anti-VANGL1 antibody [CL0241] (ab242311)

    Immunocytochemistry/Immunofluorescence analysis of MCF7 (Human breast adenocarcinoma cell line) cells labeling VANGL1 using ab242311 at 4 µg/ml (green). Nuclear probe is visualized in blue.

  • Immunocytochemistry/ Immunofluorescence - Anti-VANGL1 antibody [CL0241] (ab242311)
    Immunocytochemistry/ Immunofluorescence - Anti-VANGL1 antibody [CL0241] (ab242311)

    Immunocytochemistry/Immunofluorescence analysis of A431 (Human epidermoid carcinoma cell line) cells labeling VANGL1 using ab242311 at 4 µg/ml (green). Nuclear probe is visualized in blue, microtubules in red.

  • Western blot - Anti-VANGL1 antibody [CL0241] (ab242311)
    Western blot - Anti-VANGL1 antibody [CL0241] (ab242311)
    Anti-VANGL1 antibody [CL0241] (ab242311) at 1 µg/ml + MCF7 (Human breast adenocarcinoma cell line) cell lysate
  • Immunocytochemistry/ Immunofluorescence - Anti-VANGL1 antibody [CL0241] (ab242311)
    Immunocytochemistry/ Immunofluorescence - Anti-VANGL1 antibody [CL0241] (ab242311)

    Immunocytochemistry/Immunofluorescence analysis of A431 (Human epidermoid carcinoma cell line) cells labeling VANGL1 using ab242311 at 4 µg/ml (green). Nuclear probe is visualized in blue.

  • Western blot - Anti-VANGL1 antibody [CL0241] (ab242311)
    Western blot - Anti-VANGL1 antibody [CL0241] (ab242311)
    All lanes : Anti-VANGL1 antibody [CL0241] (ab242311) at 1 µg/ml

    Lane 1 : Vector only transfected HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate
    Lane 2 : VANGL1 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag, ~3.1 kDa) in HEK-293T cell lysate
  • Western blot - Anti-VANGL1 antibody [CL0241] (ab242311)
    Western blot - Anti-VANGL1 antibody [CL0241] (ab242311)
    All lanes : Anti-VANGL1 antibody [CL0241] (ab242311) at 1 µg/ml

    Lane 1 : siRNA control transfected MCF7 (Human breast adenocarcinoma cell line) lysate
    Lane 2 : siRNA#1 transfected MCF7 lysate
    Lane 3 : siRNA#2 transfected MCF7 lysate


    Loading control: anti-PPIB.

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